ArticleScientific reports2020
Structure and function of bacteriophage CBA120 ORF211 (TSP2), the determinant of phage specificity towards E. coli O157:H7.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
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- Generating STEC-SpecificViruses · 2025Article
- RBPseg: Toward a complete phage tail fiber structure atlas.Science advances · 2025Article
- Phage-derived proteins: Advancing food safety through biocontrol and detection of foodborne pathogens.Comprehensive reviews in food science and food safety · 2025Review
- Origin, Evolution and Diversity of φ29-like Phages-Review and Bioinformatic Analysis.International journal of molecular sciences · 2024Review
- Renewed insights into Ackermannviridae phage biology and applications.Npj viruses · 2024Review
- Lytic Capsule-SpecificViruses · 2024Article
- Meeting Report of the Second Symposium of the Belgian Society for Viruses of Microbes and Launch of the Phage Valley.Viruses · 2024Article
- Genomic and Proteomic Analysis of Six Vi01-like Phages Reveals Wide Host Range and Multiple Tail Spike Proteins.Viruses · 2024Article
- Genome sequence ofMicrobiology resource announcements · 2023Article
- Phage tailspike modularity and horizontal gene transfer reveals specificity towards E. coli O-antigen serogroups.Virology journal · 2023Article
- Insights into theInternational journal of molecular sciences · 2023Article
- Tailoring the Host Range ofViruses · 2023Article
- Structure of Escherichia coli O157:H7 bacteriophage CBA120 tailspike protein 4 baseplate anchor and tailspike assembly domains (TSP4-N).Scientific reports · 2022Article
- Computational models in the service of X-ray and cryo-electron microscopy structure determination.Proteins · 2021Article
- Characterization of a novel phage depolymerase specific to Escherichia coli O157:H7 and biofilm control on abiotic surfaces.Journal of microbiology (Seoul, Korea) · 2021Article
- Structural and functional characterization of the receptor binding proteins ofComputational and structural biotechnology journal · 2021Article
- Subtypes of tail spike proteins predicts the host range ofComputational and structural biotechnology journal · 2021Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The genome of Escherichia coli O157:H7 bacteriophage vB_EcoM_CBA120 encodes four distinct tailspike proteins (TSPs). The four TSPs, TSP1-4, attach to the phage baseplate forming a branched structure. We report the 1.9 Å resolution crystal structure of TSP2 (ORF211), the TSP that confers phage specificity towards E. coli O157:H7. The structure shows that the N-terminal 168 residues involved in TSPs complex assembly are disordered in the absence of partner proteins. The ensuing head domain contains only the first of two fold modules seen in other phage vB_EcoM_CBA120 TSPs. The catalytic site resides in a cleft at the interface between adjacent trimer subunits, where Asp506, Glu568, and Asp571 are located in close proximity. Replacement of Asp506 and Asp571 for alanine residues abolishes enzyme activity, thus identifying the acid/base catalytic machinery. However, activity remains intact when Asp506 and Asp571 are mutated into asparagine residues. Analysis of additional site-directed mutants in the background of the D506N:D571N mutant suggests engagement of an alternative catalytic apparatus comprising Glu568 and Tyr623. Finally, we demonstrate the catalytic role of two interacting glutamate residues of TSP1, located in a cleft between two trimer subunits, Glu456 and Glu483, underscoring the diversity of the catalytic apparatus employed by phage vB_EcoM_CBA120 TSPs.
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