ArticleBioengineering (Basel, Switzerland)2020
Optimization of Co-Culture Conditions for a Human Vascularized Adipose Tissue Model.
Article in Bioengineering (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- A Microphysiological Interface of Skeletal Myobundles and Inflamed Adipose Tissue for Recapitulating Muscle Dysfunction in an Obese Microenvironment.Advanced healthcare materials · 2026Article
- Influence of Scaffold Structure and Biomimetic Properties on Adipose Stem Cell Homing in Personalized Reconstructive Medicine.Biomimetics (Basel, Switzerland) · 2025Review
- Biomimetic integration of functionally controlled modular tissue building blocks for engineering 3D vascularized adipose tissue.Bioactive materials · 2025Article
- Article
- Mesenchymal Stem Cell Spheroids: A Promising Tool for Vascularized Tissue Regeneration.Tissue engineering and regenerative medicine · 2024Review
- Development of Organs-on-Chips and Their Impact on Precision Medicine and Advanced System Simulation.Pharmaceutics · 2023Review
- Review
- Article
- Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments.Journal of visualized experiments : JoVE · 2023Article
- Construction of vascularized tissue-engineered breast with dual angiogenic and adipogenic micro-tissues.Materials today. Bio · 2023Article
- Improving Fat Transplantation Survival and Vascularization with Adenovirus E4+ Endothelial Cell-Assisted Lipotransfer.Cells, tissues, organs · 2023Article
- Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches.Life (Basel, Switzerland) · 2022Review
- Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks.Gels (Basel, Switzerland) · 2022Article
- Autologous Human Immunocompetent White Adipose Tissue-on-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- cytoNet: Spatiotemporal network analysis of cell communities.PLoS computational biology · 2022Article
- 3D bioprinted white adipose model forBiofabrication · 2022Article
- Effects of collagen membranes and bone substitute differ in periodontal ligament cell microtissues and monolayers.Journal of periodontology · 2022Article
- Engineering Human Beige Adipose Tissue.Frontiers in bioengineering and biotechnology · 2022Article
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- Identifying Candidate Biomarkers of Ionizing Radiation in Human Pulmonary Microvascular Lumens Using Microfluidics-A Pilot Study.Micromachines · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
In vitro adipose tissue models can be used to provide insight into fundamental aspects of adipose physiology. These systems may serve as replacements for animal models, which are often poor predictors of obesity and metabolic diseases in humans. Adipose tissue consists of a rich vasculature that is essential to its function. However, the study of endothelial cell-adipocyte interactions has been challenging due to differences in culture conditions required for the survival and function of each cell type. To address this issue, we performed an extensive evaluation of the cell culture media composition to identify the conditions optimal for the co-culture of endothelial cells and adipocytes. The effects of individual media factors on cell survival, proliferation, and differentiation were systematically explored. Several media factors were determined to disrupt the co-culture system. Optimized culture conditions were identified and used to generate a vascularized human adipose microtissue. An interconnected vascular network was established within an adipose micro-tissue, and the networks were anastomosed with perfused channels to form a functional network. In conclusion, media conditions were identified that enabled endothelial cell-adipocyte co-culture and were used to support the formation of a vascularized adipose tissue within a microfluidic device.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.