Evidence map›Paper›PMID 32956351›Full record

ArticlePloS one2020

Spontaneous liver disease in wild-type C57BL/6JOlaHsd mice fed semisynthetic diet.

Onne A H O Ronda, Bert J M van de Heijning, Alain de Bruin, Rachel E Thomas, Ingrid Martini, Martijn Koehorst, Albert Gerding, Mirjam H Koster, Vincent W Bloks, Angelika Jurdzinski and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Response to Spontaneous Cholemia in C57BL/6 Mice Predisposes to Liver Cancer in NASH.Cellular and molecular gastroenterology and hepatology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Onne A H O RondaDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.ORCID 0000-0002-7148-8635
Bert J M van de HeijningDanone Nutricia Research, Uppsalalaan, Utrecht, The Netherlands.
Alain de BruinDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Rachel E ThomasDutch Molecular Pathology Center, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Ingrid MartiniDepartment of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Martijn KoehorstDepartment of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Albert GerdingDepartment of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Mirjam H KosterDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Vincent W BloksDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Angelika JurdzinskiDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Niels L MulderDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Rick HavingaDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Eline M van der BeekDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Dirk-Jan ReijngoudDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Folkert KuipersDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Henkjan J VerkadeDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
University Medical Center Groningen · NLDanone (Netherlands) · NLUtrecht University · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mouse models are frequently used to study mechanisms of human diseases. Recently, we observed a spontaneous bimodal variation in liver weight in C57BL/6JOlaHsd mice fed a semisynthetic diet. We now characterized the spontaneous variation in liver weight and its relationship with parameters of hepatic lipid and bile acid (BA) metabolism. In male C57BL/6JOlaHsd mice fed AIN-93G from birth to postnatal day (PN)70, we measured plasma BA, lipids, Very low-density lipoprotein (VLDL)-triglyceride (TG) secretion, and hepatic mRNA expression patterns. Mice were sacrificed at PN21, PN42, PN63 and PN70. Liver weight distribution was bimodal at PN70. Mice could be subdivided into two nonoverlapping groups based on liver weight: 0.6 SD 0.1 g (approximately one-third of mice, small liver; SL), and 1.0 SD 0.1 g (normal liver; NL; p<0.05). Liver histology showed a higher steatosis grade, inflammation score, more mitotic figures and more fibrosis in the SL versus the NL group. Plasma BA concentration was 14-fold higher in SL (p<0.001). VLDL-TG secretion rate was lower in SL mice, both absolutely (-66%, p<0.001) and upon correction for liver weight (-44%, p<0.001). Mice that would later have the SL-phenotype showed lower food efficiency ratios during PN21-28, suggesting the cause of the SL phenotype is present at weaning (PN21). Our data show that approximately one-third of C57BL/6JOlaHsd mice fed semisynthetic diet develop spontaneous liver disease with aberrant histology and parameters of hepatic lipid, bile acid and lipoprotein metabolism. Study designs involving this mouse strain on semisynthetic diets need to take the SL phenotype into account. Plasma lipids may serve as markers for the identification of the SL phenotype.

Indexed as

Animal FeedAnimalsBile Acids and SaltsDisease Models, AnimalFatty AcidsFatty LiverFemaleHumansLipid MetabolismLipoproteins, VLDLLiverMaleMiceMice, Inbred C57BLSex FactorsTriglyceridesBile Acids and SaltsFatty AcidsLipoproteins, VLDLTriglyceridesvery low density lipoprotein triglyceride

Identifiers

PMID32956351
PMCPMC7505464
OpenAlexW3087170905

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.