Evidence map›Paper›PMID 32955895›Full record

ArticleNano letters2020

Glutathione-S-transferase Fusion Protein Nanosensor.

Ryan M Williams, Jackson D Harvey, Januka Budhathoki-Uprety, Daniel A Heller

Open access · greenAbstract read
In one paragraph

Article in Nano letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. High-throughput SWCNT NIR-II screening enables cell andbioRxiv : the preprint server for biology · 2026
    Article
  5. Near-Infrared Fluorescent Single-Walled Carbon Nanotubes for Biosensing.Small (Weinheim an der Bergstrasse, Germany) · 2025
    Review
  6. Article
  7. Article
  8. Review
  9. Review
  10. Biosensing with Fluorescent Carbon Nanotubes.Angewandte Chemie (International ed. in English) · 2022
    Review
  11. Optimization of ssDNA-SWCNT Ultracentrifugation via Efficacy Measurements.ECS journal of solid state science and technology : JSS · 2022
    Article
  12. Frontiers in bioengineering and biotechnology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Ryan M WilliamsMemorial Sloan Kettering Cancer Center, New York, New York 10065, United States.ORCID 0000-0002-2381-8732
Jackson D HarveyMemorial Sloan Kettering Cancer Center, New York, New York 10065, United States.ORCID 0000-0001-8700-0713
Januka Budhathoki-UpretyMemorial Sloan Kettering Cancer Center, New York, New York 10065, United States.ORCID 0000-0003-3395-4823
Daniel A HellerMemorial Sloan Kettering Cancer Center, New York, New York 10065, United States.ORCID 0000-0002-6866-0000
Memorial Sloan Kettering Cancer Center · USCity College of New York · US

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Nicholas D Socci · 1985 to 2026
$347.4M
Disparities in COVID Disease Severity and Outcomes in New York CityUL1TR002384 · NCATS · WEILL MEDICAL COLL OF CORNELL UNIV · PI JULIANNE L IMPERATO-MCGINLEY · 2017 to 2026
$86.2M
P-selectin-Mediated Targeting of PI3K Nanomedicines to the Tumor MicroenvironmentR01CA215719 · NCI · SLOAN-KETTERING INST CAN RESEARCH · PI HELLER, DANIEL ALAN · 2018 to 2022
$3.1M
Tumor-Selective Delivery Approaches for MedulloblastomaR01NS116353 · NINDS · SLOAN-KETTERING INST CAN RESEARCH · PI HELLER, DANIEL ALAN, RAJU, PRAVEEN B. · 2020 to 2024
$3.1M
Transient Metabolite Detection for Single-Cell Metabolomics and DiagnosticsDP2HD075698 · NICHD · SLOAN-KETTERING INST CAN RESEARCH · PI HELLER, DANIEL ALAN · 2012 to 2012
$2.7M
Renal tubule-specific nanotherapeutics for acute kidney injuryR01DK114321 · NIDDK · SLOAN-KETTERING INST CAN RESEARCH · PI HELLER, DANIEL ALAN, JAIMES, EDGAR A · 2018 to 2020
$796k
NCATS NIH HHS UL1 TR002384NCI NIH HHS P30 CA008748NCI NIH HHS R01 CA215719NICHD NIH HHS DP2 HD075698NIDDK NIH HHS R01 DK114321NINDS NIH HHS R01 NS116353
6 · The paper itself

Abstract

Fusion protein tags are widely used to capture and track proteins in research and industrial bioreactor processes. Quantifying fusion-tagged proteins normally requires several purification steps coupled with classical protein assays. Here, we developed a broadly applicable nanosensor platform that quantifies glutathione-S-transferase (GST) fusion proteins in real-time. We synthesized a glutathione-DNA-carbon nanotube system to investigate glutathione-GST interactions via semiconducting single-walled carbon nanotube (SWCNT) photoluminescence. We found that SWCNT fluorescence wavelength and intensity modulation occurred specifically in response to GST and GST-fusions. The sensor response was dependent on SWCNT structure, wherein

Indexed as

GlutathioneGlutathione TransferaseChromatography, AffinityProteinsGlutathioneGlutathione TransferaseProteinsaffinity tagFluorescent sensornanocarbonsolvatochromism

Identifiers

PMID32955895
PMCPMC8266418
OpenAlexW3087000431

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.