Evidence map›Paper›PMID 32955717›Full record

ArticleGenes & genomics2020

Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus).

Shuang Liang, Zhiyuan Bao, Bohao Zhao, Tong Zhou, Jiali Li, Ming Liu, Shuaishuai Hu, Naisu Yang, Yang Chen, Xinsheng Wu

Abstract read
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In one paragraph

Article in Genes & genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Characterisation of the OvineAnimals : an open access journal from MDPI · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Shuang LiangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Zhiyuan BaoCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Bohao ZhaoCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Tong ZhouCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Jiali LiCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Ming LiuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Shuaishuai HuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Naisu YangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Yang ChenCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Xinsheng WuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China. xswu@yzu.edu.cn.ORCID 0000-0001-9871-0251
Yangzhou University · CN

Funding

Modern Agricultural Industrial System Special Funding CARS-43-A-1the national key research and development program of China 2018YFD0502203
6 · The paper itself

Abstract

backgroundKeratin-associated protein (KAP), the structural protein molecule of hair fibers, plays a key role in determining the physical properties of hair. Studies of Krtap11-1 have focused only on its localization. Functional studies of Krtap11-1 in hair follicle development have so far not been reported.

objectiveThis study aimed to provide evidence for the role of Krtap11-1 in skin and hair development.

methodsFull-length cloning and analysis of Krtap11-1 were conducted to ascertain its function. Overexpression vectors and interference sequences were constructed and transfected into RAB-9 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate the hair follicle developmental stage of Krtap11-1, the expression of different tissues, and the effects on other hair follicle development-related genes.

resultsThe full length of cloned Krtap11-1 was 947 bp. Krtap11-1 was confirmed to be a hydrophilic protein localized mostly in mitochondria. The greatest mRNA expression was observed in skin. Using a follicle synchronization model, it was found that Krtap11-1 mRNA expression levels first increased then decreased over the passage of time, principally during hair follicle catagen and telogen. Following the overexpression of Krtap11-1, mRNA expression levels of the WNT-2, KRT17, BMP-2, and TGF-β-1 genes increased, and LEF-1 decreased (P < 0.05), the converse after the corresponding use of si-RNA interference.

conclusionsKrtap11-1 exerts a promoting effect. The results provide novel insight into the relationship between hair follicle development and Krtap11-1 gene expression.

Indexed as

AnimalsGene Expression Regulation, DevelopmentalHairHair FollicleKeratinsRabbitsSkinKeratinsAngora rabbitExpression analysisHair follicle developmentKrtap11-1

Identifiers

PMID32955717
OpenAlexW3087483521

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.