ArticleMicrobial biotechnology2021
Improved production of 2,3-butanediol and isobutanol by engineering electron transport chain in Escherichia coli.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Enhancing the Signature Rose Aroma ofFoods (Basel, Switzerland) · 2026Article
- Coupling genome-wide continuous perturbation with biosensor screening reveals the potential targets in yeast isopentanol synthesis network.Synthetic and systems biotechnology · 2025Article
- Engineering new-to-nature biochemical conversions by combining fermentative metabolism with respiratory modules.Nature communications · 2024Article
- Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production.Applied microbiology and biotechnology · 2024Article
- EngineeringMicroorganisms · 2023Article
- System metabolic engineering ofFrontiers in bioengineering and biotechnology · 2023Article
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
- Modulating redox metabolism to improve isobutanol production in Shimwellia blattae.Biotechnology for biofuels · 2021Article
- Improved production of 2,3-butanediol and isobutanol by engineering electron transport chain in Escherichia coli.Microbial biotechnology · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The electron transport chain (ETC) is one of the major energy generation pathways in microorganisms under aerobic condition. Higher yield of ATP can be achieved through oxidative phosphorylation with consumption of NADH than with substrate level phosphorylation. However, most value-added metabolites are in an electrochemically reduced state, which requires reducing equivalent NADH as a cofactor. Therefore, optimal production of value-added metabolites should be balanced with ETC in terms of energy production. In this study, we attempted to reduce the activity of ETC to secure availability of NADH. The ETC mutants exhibited poor growth rate and production of fermentative metabolites compared to parental strain. Introduction of heterologous pathways for synthesis of 2,3-butanediol and isobutanol to ETC mutants resulted in increased titres and yields of the metabolites. ETC mutants yielded higher NADH/NAD
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Registered trials
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