Evidence map›Paper›PMID 32954443›Full record

ArticleThe Journal of membrane biology2020

Non-detergent Isolation of Membrane Structures from Beet Plasmalemma and Tonoplast Having Lipid Composition Characteristic of Rafts.

Natalia V Ozolina, Irina S Nesterkina, Veronika V Gurina, Vadim N Nurminsky

Abstract read
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In one paragraph

Article in The Journal of membrane biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Polar Glycerolipids and Membrane Lipid Rafts.International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Natalia V OzolinaSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, 132, Lermontov st., Irkutsk, 664033, Russia.
Irina S NesterkinaSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, 132, Lermontov st., Irkutsk, 664033, Russia.
Veronika V GurinaSiberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, 132, Lermontov st., Irkutsk, 664033, Russia.
Vadim N NurminskySiberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, 132, Lermontov st., Irkutsk, 664033, Russia. vadyanurm@mail.ru.ORCID 0000-0003-1265-1639
Siberian Institute of Plant Physiology and Biochemistry · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vacuolar and plasma membranes were isolated by a detergent-free method from beet roots (Beta vulgaris L.), and were fractionated in a sucrose density gradient of 15-60% by high-speed centrifugation at 200,000×g during 18 h. The membrane material distributed over the sucrose density gradient was analyzed for the presence of lipids characteristic of raft structures in different zones of the gradient. The quantitative and qualitative content of lipids and sterols, and the composition of fatty acids were analyzed. Some membrane structures differing in their biochemical characteristics were revealed to be located in different zones of the sucrose gradient. The results of the analysis allowed us to identify three zones in the sucrose gradient after the vacuolar membrane fractionation and two zones in the plasma membrane where membrane structures, which may be defined as rafts for their lipid composition, were presented.

Indexed as

Beta vulgarisCell FractionationChemical FractionationFatty AcidsGas Chromatography-Mass SpectrometryMembrane LipidsMembrane MicrodomainsSterolsFatty AcidsMembrane LipidsSterolsMembrane lipidsPlasma membraneRaftsTonoplast

Identifiers

PMID32954443
OpenAlexW3087461272

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.