Evidence map›Paper›PMID 32953787›Full record

ArticleAnnals of translational medicine2020

Improved osteogenic activity and inhibited bacterial biofilm formation on andrographolide-loaded titania nanotubes.

Eryou Feng, Kaiwei Shen, Feitai Lin, Wentao Lin, Tao Zhang, Yiyuan Zhang, Fengfei Lin, Yun Yang, Changjian Lin

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
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Article in Annals of translational medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Eryou FengDepartment of Arthrosis Surgery, Fuzhou Second Hospital Affiliated to Xiamen University, Teaching Hospital of Fujian Medical University, Fuzhou, China.
Kaiwei ShenDepartment of Arthrosis Surgery, Fuzhou Second Hospital Affiliated to Xiamen University, Teaching Hospital of Fujian Medical University, Fuzhou, China.
Feitai LinDepartment of Arthrosis Surgery, Fuzhou Second Hospital Affiliated to Xiamen University, Teaching Hospital of Fujian Medical University, Fuzhou, China.
Wentao LinDepartment of Arthrosis Surgery, Fuzhou Second Hospital Affiliated to Xiamen University, Teaching Hospital of Fujian Medical University, Fuzhou, China.
Tao ZhangDepartment of Arthrosis Surgery, Fuzhou Second Hospital Affiliated to Xiamen University, Teaching Hospital of Fujian Medical University, Fuzhou, China.
Yiyuan ZhangDepartment of Arthrosis Surgery, Fuzhou Second Hospital Affiliated to Xiamen University, Teaching Hospital of Fujian Medical University, Fuzhou, China.
Fengfei LinDepartment of Orthopaedics, Fuzhou Second Hospital Affiliated to Xiamen University, Teaching Hospital of Fujian Medical University, Fuzhou, China.
Yun YangResearch Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, College of Physical Science and Technology, Xiamen University, Xiamen, China.
Changjian LinState Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.
Fujian Medical University · CNXiamen University · CNFuzhou Second Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDelivery of local drugs with a titania nanotube is an attractive approach to combat implant-related infection. Our earlier study has confirmed that nanotubes loaded with gentamicin could significantly improve the antibacterial ability. On this basis, the used andrographolide in this paper has a high antibacterial activity, which cannot only avoid the evolution of antibiotic-resistant bacteria but also has simultaneously excellent biocompatibility with osteogenic cells.

methodsTwo mg of andrographolide was loaded into titania nanotubes, which were fabricated into different diameters (50 and 100 nm) and 200 nm length by the method of lyophilization and vacuum drying. We chose a standard strain, Staphylococcus epidermidis (American Type Culture Collection 35984), and two clinical isolates, S. aureus 376 and S. epidermidis 389 to research the bacterial adhesion at 6, 12 and 24 hours and biofilm formation at 48, and 72 hours on the andrographolide-loaded nanotubes (NT-A) using the diffusion plate method. Smooth titanium (smooth Ti) and nanotubes with no drug loading (NT) were also inclusive and analyzed. Furthermore, the Sprague-Dawley (SD) rats mesenchymal stem cells were used to assess the influence of nanotubular topographies on the osteogenic differentiation of mesenchymal stem cells.

resultsOur results showed that NT-A could inhibit bacterial adhesion and biofilm formation on implant surfaces. NT-A and NT, especially those with 100 nm diameters, were found to significantly promoted cell attachment, proliferation, diffusion, and osteogenic differentiation when compared with smooth Ti, while the same diameter in NT-A and NT did not differ.

conclusionsTitania nanotube modification and andrographolide loading can significantly improve the antibacterial ability and osteogenic activity of orthopedic implants. Nanotubes-based local delivery could be a promising strategy for combating implant-associated infection.

Indexed as

Andrographolidebacteria adhesionbiofilm formationimplant-associated infectiontitania nanotubes

Identifiers

PMID32953787
PMCPMC7475475
OpenAlexW3059672367

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.