ArticleIranian journal of basic medical sciences2020
Bioinformatics prediction and experimental validation of VH antibody fragment interacting with
Article in Iranian journal of basic medical sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 4 citations in OpenAlex.
- An update on antiviral antibody-based biopharmaceuticals.International immunopharmacology · 2020Review
- Hemolytic-Pred: A machine learning-based predictor for hemolytic proteins using position and composition-based features.Digital healthArticle
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Authors and funding
7 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesWe previously conducted an MATERIALS AND
methodsIn this study, at first, the three-dimensional structure of VH fragment was simulated by Kotai Antibody Builder web server. By using ClusPro 2.0 web server, the 3D structure of the soluble form of fHbp (PDB: 2KC0) was docked to the modeled VH fragment to extract the structure of the complex's binding. Molecular dynamics (MD) simulation was carried out using GROMACS 4.5.3 package for 65 ns. Secondly, coding sequence of VH fragment was cloned separately and expressed in
resultsImportant amino acids involved in antigen- antibody interaction were identified by analyzing the fHbp-VH complex. The ability of the VH antibody fragment to bind and detect fHbp antigen has been confirmed by the results of
conclusionThese results showed that this small fragment of antibody could be used for designing diagnostic kits.
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Registered trials
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