Evidence map›Paper›PMID 32952935›Full record

ReviewComputational and structural biotechnology journal2020

Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences.

Long Chen, Xingye Chen, Xusan Yang, Chao He, Miaoyan Wang, Peng Xi, Juntao Gao

Abstract readReview
In one paragraph

Review in Computational and structural biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Long ChenDepartment of Automation, Tsinghua University, 100084 Beijing, China.
Xingye ChenDepartment of Automation, Tsinghua University, 100084 Beijing, China.
Xusan YangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Chao HeDepartment of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.
Miaoyan WangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Peng XiDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Juntao GaoDepartment of Automation, Tsinghua University, 100084 Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence polarization microscopy (FPM) analyzes both intensity and orientation of fluorescence dipole, and reflects the structural specificity of target molecules. It has become an important tool for studying protein organization, orientational order, and structural changes in cells. However, suffering from optical diffraction limit, conventional FPM has low orientation resolution and observation accuracy, as the polarization information is averaged by multiple fluorescent molecules within a diffraction-limited volume. Recently, novel super-resolution FPMs have been developed to break the diffraction barrier. In this review, we will introduce the recent progress to achieve sub-diffraction determination of dipole orientation. Biological applications, based on polarization analysis of fluorescence dipole, are also summarized, with focus on chromophore-target molecule interaction and molecular organization.

Indexed as

Biomolecule interactionsDipole orientationFluorescence polarization microscopyMolecular organizationSuper-resolution

Identifiers

PMID32952935
PMCPMC7476067

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.