ArticleThe ISME journal2021
Quorum sensing controls persistence, resuscitation, and virulence of Legionella subpopulations in biofilms.
Article in The ISME journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- From dividing to dormant: embracing the full activity spectrum for environmental microorganisms.Microbiology and molecular biology reviews : MMBR · 2026Review
- The sleeping bacterium: shedding light on the resuscitation mechanism.European biophysics journal : EBJ · 2026Article
- Measuring Communication in Microbial Biofilms in Response to Antibiotics, Phytochemicals and Stressors.Antioxidants (Basel, Switzerland) · 2026Article
- Regulation of ribosome hibernation controlsmBio · 2026Article
- A bacterial toxin-antitoxin system involved in an unusual response to genotoxic stress.EMBO reports · 2025Article
- Inter-kingdom signaling by the Legionella autoinducer LAI-1 involves the antimicrobial guanylate binding protein GBP.PLoS pathogens · 2025Article
- Novel approaches on root canal disinfection methods againstJournal of oral microbiology · 2025Review
- Analysis of the genes and mechanisms responsible for the cytotoxicity of theFrontiers in microbiology · 2025Article
- Small molecule communication ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Article
- Ecology ofBiofilm · 2024Review
- Protozoan predation enhances stress resistance and antibiotic tolerance in Burkholderia cenocepacia by triggering the SOS response.The ISME journal · 2024Article
- Phagotrophic protists preserve antibiotic-resistant opportunistic human pathogens in the vegetable phyllosphere.ISME communications · 2023Article
- Review
- A clash of quorum sensing vs quorum sensing inhibitors: an overview and risk of resistance.Archives of microbiology · 2023Review
- Intracellular persister: A stealth agent recalcitrant to antibiotics.Frontiers in cellular and infection microbiology · 2023Review
- Bacterial persistence inFrontiers in cellular and infection microbiology · 2023Article
- Novel Insights into Microbial Behavior Gleaned Using Microfluidics.Microbes and environments · 2023Review
- The Effect Citrox BCL onAntioxidants (Basel, Switzerland) · 2022Article
- Methods to monitor bacterial growth and replicative rates at the single-cell level.FEMS microbiology reviews · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The water-borne bacterium Legionella pneumophila is the causative agent of Legionnaires' disease. In the environment, the opportunistic pathogen colonizes different niches, including free-living protozoa and biofilms. The physiological state(s) of sessile Legionella in biofilms and their functional consequences are not well understood. Using single-cell techniques and fluorescent growth rate probes as well as promoter reporters, we show here that sessile L. pneumophila exhibits phenotypic heterogeneity and adopts growing and nongrowing ("dormant") states in biofilms and microcolonies. Phenotypic heterogeneity is controlled by the Legionella quorum sensing (Lqs) system, the transcription factor LvbR, and the temperature. The Lqs system and LvbR determine the ratio between growing and nongrowing sessile subpopulations, as well as the frequency of growth resumption ("resuscitation") and microcolony formation of individual bacteria. Nongrowing L. pneumophila cells are metabolically active, express virulence genes and show tolerance toward antibiotics. Therefore, these sessile nongrowers are persisters. Taken together, the Lqs system, LvbR and the temperature control the phenotypic heterogeneity of sessile L. pneumophila, and these factors regulate the formation of a distinct subpopulation of nongrowing, antibiotic tolerant, virulent persisters. Hence, the biofilm niche of L. pneumophila has a profound impact on the ecology and virulence of this opportunistic pathogen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.