Evidence map›Paper›PMID 32950972›Full record

ArticleAging2020

MiR-520d-5p modulates chondrogenesis and chondrocyte metabolism through targeting HDAC1.

Jiajia Lu, Zhibin Zhou, Bin Sun, Bin Han, Qiang Fu, Yaguang Han, Wang Yuan, Zeng Xu, Aimin Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Aging, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jiajia LuDepartment of Orthopedics and Trauma Surgery, Changzheng Hospital, Shanghai, P. R. of China.
Zhibin ZhouDepartment of Orthopedics and Trauma Surgery, Changzheng Hospital, Shanghai, P. R. of China.
Bin SunDepartment of Orthopedics and Trauma Surgery, Changzheng Hospital, Shanghai, P. R. of China.
Bin HanDepartment of Orthopedics and Trauma Surgery, Changzheng Hospital, Shanghai, P. R. of China.
Qiang FuDepartment of Orthopedics and Trauma Surgery, Changzheng Hospital, Shanghai, P. R. of China.
Yaguang HanDepartment of Orthopedics and Trauma Surgery, Changzheng Hospital, Shanghai, P. R. of China.
Wang YuanDepartment of Medicinal and Materials, General Hospital of Northern Theater Command, Shenyang, P. R. of China.
Zeng XuDepartment of Orthopedics and Trauma Surgery, Changzheng Hospital, Shanghai, P. R. of China.
Aimin ChenDepartment of Orthopedics and Trauma Surgery, Changzheng Hospital, Shanghai, P. R. of China.
Shanghai Changzheng Hospital · CNGeneral Hospital of Shenyang Military Region · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) play an essential role in the chondrogenesis and the progression of osteoarthritis (OA). This study aimed to determine miRNAs associated with chondrogenesis of human mesenchymal stem cells (hMSCs) and chondrocyte metabolism. MiRNAs were screened in hMSCs during chondrogenesis by RNA-seq and qRT-PCR. MiRNA expression was determined in primary human chondrocytes (PHCs), and degraded cartilage samples. MiRNA mimics and inhibitors were transfected to cells to determine the effect of miRNA. Bioinformatic analysis and luciferase reporter assays were applied to determine the target gene of miRNA. The results demonstrated that miR-520d-5p was increased in hMSCs chondrogenesis. The overexpression and knockdown of miR-520d-5p promoted and inhibited chondrogenesis, and regulated chondrocyte metabolism. Histone deacetylase 1 (HDAC1) was decreased in hMSCs chondrogenesis, and HDAC1 was a targeting gene of miR-520d-5p. CI994, HDAC1 inhibitor, elevated cartilage-specific gene expressions and promoted hMSCs chondrogenesis. In IL-1β-treated PHCs, CI994 promoted AGGRECAN expression and suppressed MMP-13 expression, abolishing the effect of IL-1β on PHCs. Taken together, these results suggest that miR-520d-5p promotes hMSCs chondrogenesis and regulates chondrocyte metabolism through targeting HDAC1. This study provides novel understanding of the molecular mechanism of OA progression.

Indexed as

chondrocytes metabolismchondrogenesishuman mesenchymal stem cellsmiR-520d-5posteoarthritis

Identifiers

PMID32950972
PMCPMC7585120
OpenAlexW3087312595

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.