Evidence map›Paper›PMID 32949493›Full record

ArticleMolecular cell2020

Active Genetic Neutralizing Elements for Halting or Deleting Gene Drives.

Xiang-Ru Shannon Xu, Emily A Bulger, Valentino M Gantz, Carissa Klanseck, Stephanie R Heimler, Ankush Auradkar, Jared B Bennett, Lauren Ashley Miller, Sarah Leahy, Sara Sanz Juste and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 72 citations in OpenAlex.

  1. Article
  2. Article
  3. Advances in population-based interventions to control falciparum malaria.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Xiang-Ru Shannon XuSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
Emily A BulgerSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA; Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco, and Gladstone Institutes, San Francisco, CA, USA.
Valentino M GantzSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
Carissa KlanseckSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
Stephanie R HeimlerSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
Ankush AuradkarSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
Jared B BennettBiophysics Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA.
Lauren Ashley MillerSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
Sarah LeahySection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA.
Sara Sanz JusteSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
Anna BuchmanSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
Omar S AkbariSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA.
John M MarshallDivision of Epidemiology and Biostatistics, School of Public Health, University of California, Berkeley, Berkeley, CA, USA; Innovative Genomics Institute, Berkeley, CA, USA.
Ethan BierSection of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, USA; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, CA, USA. Electronic address: ebier@ucsd.edu.
University of California San Diego · USGladstone Institutes · USInnovative Genomics Institute · USUniversity of California, Berkeley · US

Funding

The mutagenic chain reaction: a method for autocatalyic gene disseminationR01GM117321 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BIER, ETHAN · 2016 to 2024
$2.5M
Development, characterization and application of CRISPR/Cas9 gene drive technologies and related active genetic elements to benefit research and society at largeDP5OD023098 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GANTZ, VALENTINO MATTEO · 2016 to 2020
$1.9M
NIGMS NIH HHS R01 GM117321NIH HHS DP5 OD023098
6 · The paper itself

Abstract

CRISPR-Cas9-based gene drive systems possess the inherent capacity to spread progressively throughout target populations. Here we describe two self-copying (or active) guide RNA-only genetic elements, called e-CHACRs and ERACRs. These elements use Cas9 produced in trans by a gene drive either to inactivate the cas9 transgene (e-CHACRs) or to delete and replace the gene drive (ERACRs). e-CHACRs can be inserted at various genomic locations and carry two or more gRNAs, the first copying the e-CHACR and the second mutating and inactivating the cas9 transgene. Alternatively, ERACRs are inserted at the same genomic location as a gene drive, carrying two gRNAs that cut on either side of the gene drive to excise it. e-CHACRs efficiently inactivate Cas9 and can drive to completion in cage experiments. Similarly, ERACRs, particularly those carrying a recoded cDNA-restoring endogenous gene activity, can drive reliably to fully replace a gene drive. We compare the strengths of these two systems.

Indexed as

Gene DeletionGene Drive TechnologyAnimalsChromosomesCRISPR-Associated Protein 9Drosophila melanogasterFemaleGreen Fluorescent ProteinsInheritance PatternsMutagenesisRNA, Guide, CRISPR-Cas SystemsTransgenesCRISPR-Associated Protein 9Green Fluorescent ProteinsRNA, Guide, CRISPR-Cas Systemsactive geneticsCRISPRdrive-neutralizingDrosophilae-CHACRERACRgene driveMCRmodelingrisk management

Identifiers

PMID32949493
PMCPMC10962758
OpenAlexW3087668557

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.