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ArticleMolecular biology reports2020

Assessment of genetic diversity of cultivated and wild Iranian grape germplasm using retrotransposon-microsatellite amplified polymorphism (REMAP) markers and pomological traits.

M Razi, M E Amiri, R Darvishzadeh, H Doulati Baneh, H Alipour, P Martínez-Gómez

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Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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3 citing papers in PubMed.

  1. Calcium-Binding Protein and Polymorphism inCurrent issues in molecular biology · 2024
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5 · Who and what money

Authors and funding

6 authors.

M RaziDepartment of Horticulture, University of Zanjan, P.O. Box 45371-38791, Zanjan, Iran.
M E AmiriDepartment of Horticulture, University of Zanjan, P.O. Box 45371-38791, Zanjan, Iran.
R DarvishzadehInstitute of Biotechnology, Urmia University, Shahid Beheshti St, Urmia, Iran. r.darvishzadeh@urmia.ac.ir.
H Doulati BanehHorticultural Crops Research Department, Kurdistan Agricultural and Natural Resources Research and Education Center, AREEO, Sanandaj, Iran.
H AlipourDepartment of Plant Production and Genetics, Urmia University, P.O. Box 165, 11 Kilometer Sero Road, Daneshgah Boulevard, Urmia, Iran.
P Martínez-GómezPlant Breeding Department, CEBAS-CSIC, P.O. Box 164, 30100, Espinardo, Murcia, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding the genetic diversity and relationships between genotypes is an effective step in designing effective breeding programs. Insertional polymorphisms of retrotransposons were studied in 75 cultivated and wild grape genotypes using retrotransposon-microsatellite amplified polymorphism (REMAP) technique. In the morphological part of work, seven pomological traits with a high breeding interest were also analyzed in the cultivated genotypes. A total of 328 markers were produced by 42 primer pairs, out of which 313 markers (95.43%) were polymorphic. Number of markers ranged from 4 in loci Tvv1Fa-873, Vine1-811, Gret1Ra-855 and Tvv1Fa-890 to 12 in locus Vine1Ra-841 with an average value of 7.45. Similarity values based on Dice's coefficient among all 75 grapevine genotypes varied from 0.41 to 0.77. Classification of genotypes using unweighted pair-group method using complete-linkage clustering led to six distinct groups. Some wild and cultivated varieties placed in the same groups. It seems there are close relationship between wild and cultivated genotypes and maybe wild genotypes are ancestor of native grapevines. Grouping of grapevine genotypes based on molecular marker data was not in agreement with clustering by agro-morphological data indicating that the most of multiplied sequences are confined to the non-coding regions of transposon elements. Results showed a substantial level of genetic diversity at molecular and pomological level and the potential of this diversity for future grape breeding programs.

Indexed as

Microsatellite RepeatsPolymorphism, GeneticQuantitative Trait, HeritableDNA, PlantIranVitisDNA, PlantCultivated germplasmGenetic diversityPolymorphismREMAPVitis viniferaWild germplasm

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.