ArticleApplied microbiology and biotechnology2020
High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors.
Article in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- An Aryl Alcohol Oxidase-Peroxygenase Catalyst System Enables Efficient Alcohol to Aldehyde Transformation.Organic process research & development · 2026Article
- Plant Biomass Depolymerization by Oxidoreductases: Structural Characteristics, Synergy Mechanism, and Evolutionary Adaptation.Journal of agricultural and food chemistry · 2026Review
- Enhancement of enzymatic activity by biomolecular condensates through pH buffering.Nature communications · 2025Article
- Aryl-alcohol oxidases: catalysis, diversity, structure-function and emerging biotechnological applications.Applied microbiology and biotechnology · 2025Review
- Discovery, characterization, and synthetic potential of two novel bacterial aryl-alcohol oxidases.Applied microbiology and biotechnology · 2024Article
- Identification of redox activators for continuous reactivation of glyoxal oxidase from Trametes versicolor in a two-enzyme reaction cascade.Scientific reports · 2024Article
- Photoregulation of the biosynthetic activity of the edible medicinal mushroom Lentinula edodes in vitro.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- Improvement in the Stability and Enzymatic Activity ofLife (Basel, Switzerland) · 2024Article
- Advances in the Fabrication of Nanoporous Anodic Aluminum Oxide and Its Applications to Sensors: A Review.Nanomaterials (Basel, Switzerland) · 2023Review
- Enzymatic reactions towards aldehydes: An overview.Flavour and fragrance journal · 2023Review
- Oxidation of 5-hydroxymethylfurfural with a novel aryl alcohol oxidase from Mycobacterium sp. MS1601.Microbial biotechnology · 2022Article
- Antiviral activity of mink interferon alpha expressed in the yeastFrontiers in veterinary science · 2022Article
- Characterization of a thermotolerant aryl-alcohol oxidase from Moesziomyces antarcticus oxidizing 5-hydroxymethyl-2-furancarboxylic acid.Applied microbiology and biotechnology · 2021Article
- Two adjacent C-terminal mutations enable expression of aryl-alcohol oxidase from Pleurotus eryngii in Pichia pastoris.Applied microbiology and biotechnology · 2021Article
- Co-Oxidative Transformation of Piperine to Piperonal and 3,4-Methylenedioxycinnamaldehyde by a Lipoxygenase from Pleurotus sapidus.Chembiochem : a European journal of chemical biology · 2021Article
- Extracellular Oxidase from the Neonothopanus nambi Fungus as a Promising Enzyme for Analytical Applications.The protein journal · 2021Article
- Optimizing operational parameters for the enzymatic production of furandicarboxylic acid building block.Microbial cell factories · 2021Article
- Pecularities and applications of aryl-alcohol oxidases from fungi.Applied microbiology and biotechnology · 2021Review
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Abstract
The fungal secretome comprises various oxidative enzymes participating in the degradation of lignocellulosic biomass as a central step in carbon recycling. Among the secreted enzymes, aryl-alcohol oxidases (AAOs) are of interest for biotechnological applications including production of bio-based precursors for plastics, bioactive compounds, and flavors and fragrances. Aryl-alcohol oxidase 2 (PeAAO2) from the fungus Pleurotus eryngii was heterologously expressed and secreted at one of the highest yields reported so far of 315 mg/l using the methylotrophic yeast Pichia pastoris (recently reclassified as Komagataella phaffii). The glycosylated PeAAO2 exhibited a high stability in a broad pH range between pH 3.0 and 9.0 and high thermal stability up to 55 °C. Substrate screening with 41 compounds revealed that PeAAO2 oxidized typical AAO substrates like p-anisyl alcohol, veratryl alcohol, and trans,trans-2,4-hexadienol with up to 8-fold higher activity than benzyl alcohol. Several compounds not yet reported as substrates for AAOs were oxidized by PeAAO2 as well. Among them, cumic alcohol and piperonyl alcohol were oxidized to cuminaldehyde and piperonal with high catalytic efficiencies of 84.1 and 600.2 mM
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