ArticleJournal of bacteriology1987
Association of thioredoxin with the inner membrane and adhesion sites in Escherichia coli.
Article in Journal of bacteriology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Synchrotron-radiation vacuum-ultraviolet circular dichroism spectroscopy in structural biology: an overview.Biophysics and physicobiology · 2019Review
- Construction of leaky strains and extracellular production of exogenous proteins in recombinant Escherichia coli.Microbial biotechnology · 2014Article
- Ultrastructural analysis of the rugose cell envelope of a member of the Pasteurellaceae family.Journal of bacteriology · 2013Article
- Cytosolic proteins contribute to surface plasminogen recruitment of Neisseria meningitidis.Journal of bacteriology · 2007Article
- Molecular sieve mechanism of selective release of cytoplasmic proteins by osmotically shocked Escherichia coli.Journal of bacteriology · 2001Article
- Membrane association of the Escherichia coli enterobactin synthase proteins EntB/G, EntE, and EntF.Journal of bacteriology · 2000Article
- Cyanobacterial cell walls: news from an unusual prokaryotic envelope.Journal of bacteriology · 2000Review
- Elongation factor Tu and DnaK are transferred from the cytoplasm to the periplasm of Escherichia coli during osmotic downshock presumably via the mechanosensitive channel mscL.Journal of bacteriology · 2000Article
- The reductive enzyme thioredoxin 1 acts as an oxidant when it is exported to the Escherichia coli periplasm.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Localization of DnaK (chaperone 70) from Escherichia coli in an osmotic-shock-sensitive compartment of the cytoplasm.Journal of bacteriology · 1994Article
- C9-mediated killing of bacterial cells by transferred C5b-8 complexes: transferred C5b-9 complexes are nonbactericidal.Infection and immunity · 1994Article
- Translocation of DNA across bacterial membranes.Microbiological reviews · 1994Review
- Location and unusual membrane topology of the immunity protein of the Escherichia coli phage T4.Journal of virology · 1993Article
- A signal sequence is not required for protein export in prlA mutants of Escherichia coli.The EMBO journal · 1993Article
- Abnormal fractionation of beta-lactamase in Escherichia coli: evidence for an interaction with the inner membrane in the absence of a leader peptide.Journal of bacteriology · 1992Article
- The mechanism of secretion of hemolysin and other polypeptides from gram-negative bacteria.Journal of bioenergetics and biomembranes · 1990Review
- Localization of penicillin-binding protein 1b in Escherichia coli: immunoelectron microscopy and immunotransfer studies.Journal of bacteriology · 1990Article
- Specific localization of the lysis protein of bacteriophage MS2 in membrane adhesion sites of Escherichia coli.Journal of bacteriology · 1989Article
- Localization and assembly into the Escherichia coli envelope of a protein required for entry of colicin A.Journal of bacteriology · 1989Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The intracellular localization of thioredoxin in Escherichia coli was determined by immunoelectron microscopy and correlated to previous biochemical data which had suggested that thioredoxin resides at inner-outer membrane adhesion sites. Since a considerable amount of thioredoxin was lost during preparation of cells for electron microscopy, we immobilized the protein with the heterobifunctional photoactivatable cross-linker p-azidophenacylbromide before the cells were fixed with aldehyde and embedded in Lowicryl K4M. Thin sections were labeled with affinity-purified antithioredoxin antiserum and protein A-gold complexes. Densities of immunolabel in a designated membrane-associated area and in the rest of the cytoplasm were compared and the data were statistically evaluated. Wild-type strain W3110 and strain SK3981, an overproducer of thioredoxin, exhibited increased labeling at the inner membrane and its adjacent cytoplasmic area. In contrast, the more centrally located cytoplasm of both strains showed much lower label density. This label distribution did not change with cell growth or in the stationary phase. Immunolabel was often found at bridges between the inner and outer membranes; this result is consistent with a model which places at least a portion of the thioredoxin at membrane adhesion sites, corresponding to an osmotically sensitive cytoplasmic compartment bounded by a hybrid inner-outer membrane (C.A. Lunn and V. Pigiet, J. Biol. Chem. 257:11424-11430, 1982; C.A. Lunn and V. Pigiet, J. Biol. Chem. 261:832-838, 1986). Specific label was absent in the periplasmic space.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.