Evidence map›Paper›PMID 32944865›Full record

ArticleBioprocess and biosystems engineering2021

Efficient biosynthesis of polysaccharide welan gum in heat shock protein-overproducing Sphingomonas sp. via temperature-dependent strategy.

Ping Zhu, Yijing Zhan, Cheng Wang, Xiaoliu Liu, Liming Liu, Hong Xu

Abstract read
PubMed Publisher
In one paragraph

Article in Bioprocess and biosystems engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. A nostoxanthin-producing bacterium,Frontiers in microbiology · 2023
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ping Zhu *State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, No.30 South Puzhu Road, Pukou District, Nanjing, 211816, China.
Yijing Zhan *State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, No.30 South Puzhu Road, Pukou District, Nanjing, 211816, China.
Cheng WangState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, No.30 South Puzhu Road, Pukou District, Nanjing, 211816, China.
Xiaoliu LiuState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, No.30 South Puzhu Road, Pukou District, Nanjing, 211816, China.
Liming LiuState Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China. mingll@jiangnan.edu.cn.
Hong XuState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, No.30 South Puzhu Road, Pukou District, Nanjing, 211816, China. xuh@njut.edu.cn.
Nanjing Tech University · CNJiangnan University · CN

Funding

National Natural Science Foundation of China 21776133Natural Science Foundation of Jiangsu Province BK20160985
6 · The paper itself

Abstract

Cell growth and product formation are two critical processes in polysaccharide welan biosynthesis, but the conflict between them is often encountered. In this study, a temperature-dependent strategy was designed for two-stage welan production through overexpressing heat shock proteins in Sphingomonas sp. The first stage was cell growth phase with higher TCA cycle activity at 42 °C; the second stage was welan formation phase with higher precursor synthesis pathway activity at 37 °C. The highest welan concentration 37.5 g/L was achieved after two-stage process. Ultimately, this strategy accumulated welan yield of 79.2 g/100 g glucose and productivity of 0.62 g/L/h at 60 h, which were the best reported results so far. The duration of fermentation was shortened. Besides, rheological behavior of welan gum solutions remained stable at wide range of temperature, pH, and NaCl. These results indicated that this approach efficiently improved welan synthesis.

Indexed as

Bacterial ProteinsHeat-Shock ProteinsHot TemperaturePolysaccharides, BacterialSphingomonasBacterial ProteinsHeat-Shock ProteinsPolysaccharides, BacterialwelanBiosynthesisHeat shock proteinTemperature dependentTwo-stage fermentationWelan gum

Identifiers

PMID32944865
OpenAlexW3087736672

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.