ArticlePlant methods2020
An improved pollen number counting method using a cell counter and mesh columns.
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- A single-nucleotide substitution ofPNAS nexus · 2023Article
- Pollen production of downy birch (Betula pubescens Ehrh.) along an altitudinal gradient in the European Alps.International journal of biometeorology · 2023Article
- Development and application of a method to classify airborne pollen taxa concentration using light scattering data.Scientific reports · 2021Article
- Factors Affecting the Number of Pollen Grains per Male Strobilus in Japanese Cedar (Plants (Basel, Switzerland) · 2021Article
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4 authors.
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Abstract
backgroundThe determination of pollen number is important in evolutionary, agricultural, and medical studies. Tree species of the Cupressaceae family cause serious pollinosis worldwide. Although Japanese cedar (
resultsHere, we report an improved pollen counting method for Japanese cedar using a precise and rapid cell counter in combination with home-made mesh columns. The male strobilus was gently crushed using a pestle. Large and small debris were then removed using 100- and 20-μm mesh columns, respectively. We successfully detected pollen sizes and numbers that differed between two clones using this method.
conclusionsThis improved method is not only suitable for counting pollen from Japanese cedar, but could also be applied to other species of the Cupressaceae family with hard scale tissue covering the pollen. Moreover, this method could be applied to a broader range of plant species, such as wheat, because there is no need to wait for anthesis and debris can be removed efficiently.
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