Evidence map›Paper›PMID 32943007›Full record

ArticleBMC microbiology2020

EttA is likely non-essential in Staphylococcus aureus persistence, fitness or resistance to antibiotics.

Michal Meir, Anna Rozenblit, Simona Fliger, Yuval Geffen, Daniel Barkan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 62% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Michal MeirThe Ruth Rappaport Children's Hospital, Rambam Health Care Campus, Haifa, Israel. MI_MEIR@rambam.health.gov.il.
Anna RozenblitKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Simona FligerKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Yuval GeffenClinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel.
Daniel BarkanKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Hebrew University of Jerusalem · ILRambam Health Care Campus · IL

Funding

Israeli Science Foundation 739/19
6 · The paper itself

Abstract

backgroundTolerance to antibiotics and persistence are associated with antibiotic treatment failures, chronic-relapsing infections, and emerging antibiotic resistance in various bacteria, including Staphylococcus aureus. Mechanisms of persistence are largely unknown, yet have been linked to physiology under low-ATP conditions and the metabolic-inactive state. EttA is an ATP-binding cassette protein, linked in Eschrechia coli to ribosomal hibernation and fitness in stationary growth phase, yet its role in S. aureus physiology is unknown.

resultsUsing whole genome sequencing (WGS) of serial clinical isolates, we identified an EttA-negative S. aureus mutant (ettA

conclusionsLack of ettA does not affect S. aureus antibiotic resistance, beta-lactam tolerance, resilience to starvation or fitness following starvation. We conclude the role of ettA in S. aureus physiology is limited or redundant with another, unidentified gene. WGS of serial clinical isolates may enable investigation of other single genes involved in S. aureus virulence, and specifically persister cell formation.

Indexed as

Genetic FitnessGenome, BacterialAnti-Bacterial AgentsATP-Binding Cassette TransportersBacterial ProteinsCefazolinClone CellsCulture MediaDrug Resistance, Multiple, BacterialGene ExpressionHumansMicrobial Sensitivity TestsStaphylococcal InfectionsStaphylococcus aureusVirulenceWhole Genome SequencingAnti-Bacterial AgentsATP-Binding Cassette TransportersBacterial ProteinsCefazolinCulture MediaAntibiotic resistanceAntibiotic toleranceFitnessKilling curvePersistenceRibosomal hibernationStaphylococcus aureus

Identifiers

PMID32943007
PMCPMC7499846
OpenAlexW3084511411

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.