ArticlePloS one2020
Screening and verification of reference genes for analysis of gene expression in winter rapeseed (Brassica rapa L.) under abiotic stress.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 42 citations in OpenAlex.
- Identification of Reference Genes for RT-qPCR Assays inCurrent issues in molecular biology · 2026Article
- Drought Stress Response of Doubled Haploid Interspecific Rapeseed Hybrids at Germination and Flowering Stages.Biology · 2026Article
- Genome-Wide Identification and Analysis of Plant Cysteine Oxidase (International journal of molecular sciences · 2026Article
- Evaluation of reference genes for accurate qRT-PCR normalization in bitter gourd across different tissues and stress conditions.BMC plant biology · 2025Article
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- The Potential Role ofGenes · 2025Article
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- Identification and validation of stable reference genes for RT-qPCR analyses of Kobresia littledalei seedlings.BMC plant biology · 2024Article
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- Genome-Wide Identification and Analysis of the Plant Cysteine Oxidase (PCO) Gene Family inInternational journal of molecular sciences · 2023Article
- Silicon EnhancesPlants (Basel, Switzerland) · 2023Article
- Selection and verification of reliable internal reference genes in stem development of herbaceous peony (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Screening and validating of endogenous reference genes in Chlorella sp. TLD 6B under abiotic stress.Scientific reports · 2023Article
- Single-cell transcriptome reveals dominant subgenome expression and transcriptional response to heat stress in Chinese cabbage.Genome biology · 2022Article
- Regulatory interaction of BcWRKY33A and BcHSFA4A promotes salt tolerance in non-heading Chinese cabbage [Horticulture research · 2022Article
- Article
- Genome-Wide Identification and Analysis of the Ascorbate Peroxidase (APX) Gene Family of Winter Rapeseed (Frontiers in genetics · 2021Article
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Winter rapeseed (Brassica rapa L.) is the main oilseed crop in northern China and can safely overwinter at 35 (i.e., Tianshui, China) to 48 degrees north latitude (i.e., Altai, Heilongjiang, Raohe, and Xinjiang, China). In order to identify stable reference genes to understand the molecular mechanisms of stress tolerance in winter rapeseed, internal reference genes of winter rapeseed under four abiotic stresses were analyzed using GeNorm, NormFinder, BestKeeper, and RefFinder software. The most stable combinations of internal reference genes were β-actin and SAND in cold-stressed leaves, β-actin and EF1a in cold-stressed roots, F-box and SAND in high temperature-stressed leaves, and PP2A and RPL in high temperature-stressed roots, SAND and PP2A in NaCl-stressed leaves, RPL and UBC in NaCl-stressed roots, RPL and PP2A in PEG-stressed leaves, and PP2A and RPL in PEG-stressed roots. Expression profiles of PXG3 were used to verify these results. The stable reference genes identified in this study are useful tools for identifying stress-responsive genes to understand the molecular mechanisms of stress tolerance in winter rapeseed.
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