ReviewJournal of anatomy2021
Tissue clearing technique: Recent progress and biomedical applications.
Review in Journal of anatomy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
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Who cites it
73 citing papers in PubMed.
- Three-Dimensional Architecture of Ectopic Epithelium and Vasculature in Ovarian Endometriosis Revealed by Tissue-Clearing Imaging.Cell proliferation · 2026Article
- Multiscale analysis of the adult human superior hypogastric plexus and hypogastric nerve.Journal of anatomy · 2026Article
- Human organoids: Fit for drug discovery?Stem cell reports · 2026Review
- From identity to function: unveiling the cellular complexity of hypothalamic feeding circuits.Reviews in endocrine & metabolic disorders · 2026Review
- Novel fixative solutions with superior preservation of structure and antigenicity are suitable for optical clearing, tissue staining, and flow cytometry.Nagoya journal of medical science · 2026Article
- Comparative analysis of clearing methods for 3D imaging of the vasculature in mineralized mouse tissues.iScience · 2026Article
- Multidimensional lung imaging: integrated preclinical platforms enabling the identification of translational biomarkers for pulmonary research.Respiratory research · 2026Review
- Immunohistochemical Localization and Anatomical Distribution of Enteric Glia Cells in Enteric Nervous System.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
- Rapid sonication-assisted whole tissue clearing and immunostaining.Scientific reports · 2025Article
- Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Optimization of Tissue Clearing Methods and Imaging Conditions for 3D Visualization of the Vasculature of the Adult Murine Knee.bioRxiv : the preprint server for biology · 2025Article
- Improving occlusal caries detection using optical clearing agent for optical coherence tomography.Lasers in medical science · 2025Article
- A highly stable monomeric red fluorescent protein for advanced microscopy.Nature methods · 2025Article
- In Toto Adipocytes Analysis Using Hydrophilic Tissue Clearing, Light Sheet Microscopy, and Deep Learning-Based Image Processing.Biology of the cell · 2025Article
- Three-dimensional imaging and computational quantitation as a novel approach to assess nerve fibers, enteric glial cells, mast cells, and the proximity of mast cells to the nerve fibers in human sigmoid mucosal biopsies from healthy subjects.Journal of neuroscience methods · 2025Article
- Comprehensive 3D Imaging of Whole Zebrafish Using a Water-Based Clearing Reagent for Hard Tissues.Zebrafish · 2025Article
- An acoustic levitation platform for high-content histological analysis of 3D tissue culture.Lab on a chip · 2025Article
- Progress on three-dimensional visualizing skin architecture with multiple immunofluorescence staining and tissue-clearing approaches.Histology and histopathology · 2025Review
- Quantifying the relationship between cell proliferation and morphology during development of the face.Development (Cambridge, England) · 2025Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organisms are inherently three dimensional, thus comprehensive understanding of the complicated biological system requires analysis of organs or even whole bodies in the context of three dimensions. However, this is a tremendous task since the biological specimens are naturally opaque, a major obstacle in whole-body and whole-organ imaging. Tissue clearing technique provides a prospective solution and has become a powerful tool for three-dimensional imaging and quantification of organisms. Tissue clearing technique aims to make tissue transparent by minimizing light scattering and light absorption, thus allowing deep imaging of large volume samples. When combined with diverse molecular labeling methods and high-throughput optical sectioning microscopes, tissue clearing technique enables whole-body and whole-organ imaging at cellular or subcellular resolution, providing detailed and comprehensive information about the intact biological systems. Here, we give an overview of recent progress and biomedical applications of tissue clearing technique. We introduce the mechanisms and basic principles of tissue clearing, and summarize the current tissue clearing methods. Moreover, the available imaging techniques and software packages for data processing are also presented. Finally, we introduce the recent advances in applications of tissue clearing in biomedical fields. Tissue clearing contributes to the investigation of structure-function relationships in intact mammalian organs, and opens new avenues for cellular and molecular mapping of intact human organs. We hope this review contributes to a better understanding of tissue clearing technique and can help researchers to select the best-suited clearing protocol for their experiments.
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