Evidence map›Paper›PMID 32939727›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Xenopus Deep Cell Aggregates: A 3D Tissue Model for Mesenchymal-to-Epithelial Transition.

Hye Young Kim, Lance A Davidson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 2 countries.

Hye Young KimDepartment of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA, USA.
Lance A DavidsonDepartment of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA, USA. lad43@pitt.edu.
University of Pittsburgh · US

Funding

The Biomechanics of morphogenesis in the frogR01HD044750 · NICHD · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI DAVIDSON, LANCE A. · 2005 to 2020
$3.8M
Biomechanics of MorphogenesisR37HD044750 · NICHD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LANCE A. DAVIDSON · 2022 to 2026
$2.1M
Mechanical Control of Mesenchymal-to-Epithelial TransitionR56HL134195 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI DAVIDSON, LANCE A. · 2016 to 2016
$550k
NHLBI NIH HHS R56 HL134195NICHD NIH HHS R01 HD044750NICHD NIH HHS R37 HD044750
6 · The paper itself

Abstract

Mesenchymal-to-epithelial transition (MET) describes the ability of loosely associated migratory cells to form a more adherent sheet-like assembly of cells. MET is a conserved motif occurring throughout organogenesis and plays a key role in regeneration and cancer metastasis, and is the first step in producing induced pluripotent stem cells (iPSCs). To resolve fundamental biological questions about MET, its relation to epithelial-to-mesenchymal transition, and to explore MET's role in tissue assembly and remodeling requires live models for MET that are amenable to experimentation. Many cases of clinically important MET are inferred since they occur deep with the body of the embryo or adult. We have developed a tractable model for MET, where cellular transitions can be directly observed under conditions where molecular, mechanical, and cellular contexts can be controlled experimentally. In this chapter, we introduce a 3-dimensional (3D) tissue model to study MET using Xenopus laevis embryonic mesenchymal cell aggregates.

Indexed as

AnimalsCell MovementCell TrackingEctodermEpithelial CellsImaging, Three-DimensionalMesenchymal Stem CellsMesodermTissue Culture TechniquesXenopus3D aggregateDissociationImmunofluorescenceMesenchymal-to-epithelial transitionMesenchymeMicrosurgery

Identifiers

PMID32939727
PMCPMC9972462
OpenAlexW3087487818

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.