ArticleJournal of oral biology and craniofacial research
Fibroblast encapsulation in gelatin methacryloyl (GelMA) versus collagen hydrogel as substrates for oral mucosa tissue engineering.
Article in Journal of oral biology and craniofacial research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- Smart Hydrogels for Craniofacial Regeneration.Cells · 2026Review
- Modern Drug Delivery Platforms Based on Photocrosslinkable Hydrogels (PCHs) in Dentistry: From Material Characteristics to Clinical Applications-A Review.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Chemically modified gelatin derivatives: synthetic strategies, physicochemical properties, biodegradation, drug delivery and biomedical applications.Frontiers in pharmacology · 2026Review
- Advancements and applications of gelatin-based scaffolds in dental engineering: a narrative review.Odontology · 2026Review
- Next-Generation Natural Hydrogels in Oral Tissue Engineering.Pharmaceutics · 2025Review
- Development of a Photocrosslinkable Collagen-Bone Matrix Hydrogel for Bone Tissue Engineering.Polymers · 2025Article
- The Use of High-Concentration Collagen-Based Composition and Gelatin Granules as Bioinks for Extrusion 3D Bioprinting of Porous-Structured Hydrogel Constructs.Sovremennye tekhnologii v meditsine · 2025Article
- The Effect of Carbodiimide Crosslinkers on Gelatin Hydrogel as a Potential Biomaterial for Gingival Tissue Regeneration.Gels (Basel, Switzerland) · 2024Article
- Advances and Functional Integration of Hydrogel Composites as Drug Delivery Systems in Contemporary Dentistry.Gels (Basel, Switzerland) · 2024Review
- Exosome-Laden Hydrogels as Promising Carriers for Oral and Bone Tissue Engineering: Insight into Cell-Free Drug Delivery.International journal of molecular sciences · 2024Review
- Characterization of Photo-Crosslinked Methacrylated Type I Collagen as a Platform to Investigate the Lymphatic Endothelial Cell Response.Lymphatics · 2024Article
- Precision Engineering of Chondrocyte Microenvironments: Investigating the Optimal Reaction Conditions for Type B Gelatin Methacrylate Hydrogel Matrix for TC28a2 Cells.Journal of functional biomaterials · 2024Article
- UV-polymerizable methacrylated gelatin (GelMA)-based hydrogel containing tannic acids for wound healing.Drug delivery and translational research · 2023Article
- Recent trends and perspectives in reconstruction and regeneration of intra/extra-oral wounds using tissue-engineered oral mucosa equivalents.The Japanese dental science review · 2023Review
- Hydrogel-Based Biomaterial as a Scaffold for Gingival Regeneration: A Systematic Review of In Vitro Studies.Polymers · 2023Review
- Hydrogels for Oral Tissue Engineering: Challenges and Opportunities.Molecules (Basel, Switzerland) · 2023Review
- Cell-scaffold interactions in tissue engineering for oral and craniofacial reconstruction.Bioactive materials · 2023Article
- Development of fibroblast/endothelial cell-seeded collagen scaffolds for in vitro prevascularization.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Development of Scaffolds from Bio-Based Natural Materials for Tissue Regeneration Applications: A Review.Gels (Basel, Switzerland) · 2023Review
- Tissue-engineered edible bird's nests (TeeBN).International journal of bioprinting · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeOver the past decades, a variety of biomaterials have been investigated in terms of their suitability for oral mucosa tissue engineering. The aim of this study was to compare collagen and GelMA hydrogels as connective tissue scaffolds for fibroblasts and as substrates for seeding and culture of oral epithelial keratinocyte cells.
methodsHuman primary oral fibroblast and keratinocyte cells were isolated from gingival biopsies. The mixture of fibroblasts with GelMA or collagen gel were aliquoted within six-well tissue culture plate inserts and cross-linked using visible light or reconstitution buffer/heat, respectively. The viability of fibroblasts in the hydrogels was investigated after one and three days of cultivation using the PrestoBlue assay. Following the addition and culture of oral keratinocytes onto the connective tissue constructs, the tissue-engineered oral mucosa was assessed histologically.
resultsThe tissue viability assay shows that collagen hydrogels encapsulating fibroblasts displayed significantly higher cell viability than cell-laden GelMA constructs after 24 and 72 h (
conclusionCollagen-based scaffold offers superior biological properties compared to GelMA hydrogel in terms of oral fibroblast growth, as well as epithelial cell adhesion and differentiation. Therefore, collagen-based hydrogels remain the preferred choice for oral mucosa tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.