ArticleScientific reports2020
Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Enhanced Sensitivity and Human Relevance: a TNF-α-Induced HaCaT Keratinocyte Model for Screening Anti-Inflammatory Cosmetic Materials.Journal of applied toxicology : JAT · 2026Article
- Skin-Interfaced Therapeutic Patches for Wound Fluid Management and Transdermal Drug Delivery.Advanced healthcare materials · 2026Article
- HaCaT Keratinocytes: A Differentiation-Competent Platform for Episomal Replication of HPV Type 11.Viruses · 2026Article
- Transcriptomic Analysis of TDP1-Knockout HEK293A Cells Treated with the TDP1 Inhibitor (Usnic Acid Derivative).International journal of molecular sciences · 2025Article
- Exposure of cells to near-infrared irradiation relaxes chromatin compaction and facilitates recognition of cyclo-butane pyrimidine dimers.Scientific reports · 2025Article
- Phytochemical Characterization, Antioxidant Activity, and Anti-Melanoma Mechanism of Flower Buds ofPlants (Basel, Switzerland) · 2025Article
- Is Silver a Precious Metal for G-Quadruplex Stabilization Mediated by Porphyrins?International journal of molecular sciences · 2024Article
- Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation.Biomolecules · 2024Article
- Antioxidants Hydroxytyrosol and Thioredoxin-Mimetic Peptide CB3 Protect Irradiated Normal Tissue Cells.Antioxidants (Basel, Switzerland) · 2024Article
- The Ultraviolet Irradiation of Keratinocytes Induces Ectopic Expression of LINE-1 Retrotransposon Machinery and Leads to Cellular Senescence.Biomedicines · 2023Article
- Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2.Scientific reports · 2023Article
- Exploring Cell Migration Mechanisms in Cancer: From Wound Healing Assays to Cellular Automata Models.Cancers · 2023Article
- Innovative Rocuronium Bromide Topical Formulation for Targeted Skin Drug Delivery: Design, Comprehensive Characterization, In Vitro 2D/3D Human Cell Culture and Permeation.International journal of molecular sciences · 2023Article
- Are genetic drift and stem cell adherence in laboratory culture issues for cultivated meat production?Frontiers in nutrition · 2023Review
- Pitfalls in the Application of Dispase-Based Keratinocyte Dissociation Assay for In Vitro Analysis of Pemphigus Vulgaris.Vaccines · 2022Article
- Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1.Scientific reports · 2021Article
- MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents.Marine drugs · 2021Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We here present the spontaneously immortalised cell line, HaSKpw, as a novel model for the multistep process of skin carcinogenesis. HaSKpw cells were established from the epidermis of normal human adult skin that, without crisis, are now growing unrestricted and feeder-independent. At passage 22, clonal populations were established and clone7 (HaSKpwC7) was further compared to the also spontaneously immortalized HaCaT cells. As important differences, the HaSKpw cells express wild-type p53, remain pseudodiploid, and show a unique chromosomal profile with numerous complex aberrations involving chromosome 20. In addition, HaSKpw cells overexpress a pattern of genes and miRNAs such as KRT34, LOX, S100A9, miR21, and miR155; all pointing to a tumorigenic status. In concordance, HaSKpw cells exhibit reduced desmosomal contacts that provide them with increased motility and a highly migratory/invasive phenotype as demonstrated in scratch- and Boyden chamber assays. In 3D organotypic cultures, both HaCaT and HaSKpw cells form disorganized epithelia but only the HaSKpw cells show tumorcell-like invasive growth. Together, HaSKpwC7 and HaCaT cells represent two spontaneous (non-genetically engineered) "premalignant" keratinocyte lines from adult human skin that display different stages of the multistep process of skin carcinogenesis and thus represent unique models for analysing skin cancer development and progression.
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