ArticleScientific reports2020
Hemoglobin stimulates vigorous growth of Streptococcus pneumoniae and shapes the pathogen's global transcriptome.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Grassland Saline-Alkaline Degradation-Induced Excessive Iron and Sodium Intake Potentially Increases the Transmission Risk of Fecal Pathogenic Bacteria in Cattle.Animals : an open access journal from MDPI · 2025Article
- Heme-mediated selection of encapsulatedEmerging microbes & infections · 2025Article
- Transcriptomic analysis ofMicrobial genomics · 2025Article
- Time-Dependent Changes in Malondialdehyde and Free-Hemoglobin in Leukoreduced and Non-Leukoreduced Canine Packed Red Blood Cells Units During Storage.Veterinary sciences · 2025Article
- Exceptional Heme Tolerance inLife (Basel, Switzerland) · 2025Article
- Endogenously produced HMicrobiology spectrum · 2024Article
- Human monoclonal antibodies protect against viral-mediated pneumococcal superinfection.Frontiers in immunology · 2024Article
- Human Serum Supplementation Promotes Streptococcus mitis Growth and Induces Specific Transcriptomic Responses.Microbiology spectrum · 2023Article
- Oxidative Reactions Catalyzed by Hydrogen Peroxide Produced by Streptococcus pneumoniae and Other Streptococci Cause the Release and Degradation of Heme from Hemoglobin.Infection and immunity · 2022Article
- Heme-induced genes facilitate endosymbiont (Sodalis glossinidius) colonization of the tsetse fly (Glossina morsitans) midgut.PLoS neglected tropical diseases · 2022Article
- Targeted Transcriptomic Screen of Pneumococcal Genes Expressed during Murine and Human Infection.Infection and immunity · 2022Article
- Hemoglobin Induces Early and Robust Biofilm Development inInfection and immunity · 2021Article
- Media Matters, Examining Historical and ModernFrontiers in cellular and infection microbiology · 2021Review
- Virus-Induced Changes of the Respiratory Tract Environment Promote Secondary Infections WithFrontiers in cellular and infection microbiology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus pneumoniae (Spn) must acquire iron from the host to establish infection. We examined the impact of hemoglobin, the largest iron reservoir in the body, on pneumococcal physiology. Supplementation with hemoglobin allowed Spn to resume growth in an iron-deplete medium. Pneumococcal growth with hemoglobin was unusually robust, exhibiting a prolonged logarithmic growth, higher biomass, and extended viability in both iron-deplete and standard medium. We observed the hemoglobin-dependent response in multiple serotypes, but not with other host proteins, free iron, or heme. Remarkably, hemoglobin induced a sizable transcriptome remodeling, effecting virulence and metabolism in particular genes facilitating host glycoconjugates use. Accordingly, Spn was more adapted to grow on the human α - 1 acid glycoprotein as a sugar source with hemoglobin. A mutant in the hemoglobin/heme-binding protein Spbhp-37 was impaired for growth on heme and hemoglobin iron. The mutant exhibited reduced growth and iron content when grown in THYB and hemoglobin. In summary, the data show that hemoglobin is highly beneficial for Spn cultivation in vitro and suggest that hemoglobin might drive the pathogen adaptation in vivo. The hemoglobin receptor, Spbhp-37, plays a role in mediating the positive influence of hemoglobin. These novel findings provide intriguing insights into pneumococcal interactions with its obligate human host.
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