Evidence map›Paper›PMID 32937243›Full record

ArticleVeterinary parasitology2020

Comparative sequences of the Wolbachia genomes of drug-sensitive and resistant isolates of Dirofilaria immitis.

Pei-Tsz Shin, Rodrigo de Paula Baptista, Connor M O'Neill, Connor Wallis, Barbara J Reaves, Adrian J Wolstenholme

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PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary parasitology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Whole-genome reference ofCurrent research in parasitology & vector-borne diseases · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Pei-Tsz ShinDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA.
Rodrigo de Paula BaptistaCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA; Institute of Bioinformatics, University of Georgia, Athens, GA, 30602, USA.
Connor M O'NeillDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA.
Connor WallisDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA.
Barbara J ReavesDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA.
Adrian J WolstenholmeDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA. Electronic address: adrianw@uga.edu.
University of Georgia · USOffice of Infectious Diseases · US

Funding

The antifilarial activity of ivermectin and diethylcarbamizineR01AI103140 · NIAID · UNIVERSITY OF GEORGIA · PI WOLSTENHOLME, ADRIAN J · 2014 to 2016
$1.1M
Trafficking of parasitic nematode ion channelsR21AI125899 · NIAID · UNIVERSITY OF GEORGIA · PI WOLSTENHOLME, ADRIAN J · 2016 to 2017
$413k
NIAID NIH HHS R01 AI103140NIAID NIH HHS R21 AI125899
6 · The paper itself

Abstract

The recent identification of isolates of D. immitis with confirmed resistance to the macrocyclic lactone preventatives presents an opportunity for comparative genomic studies using these isolates, and examining the genetic diversity within and between them. We studied the genomes of Wolbachia endosymbionts of five isolates of D. immitis maintained at the University of Georgia. Missouri and Georgia-2 are maintained as drug susceptible isolates, and JYD-27, Yazoo-2013 and Metairie-2014 are resistant to the macrocyclic lactone preventatives. We used whole genome amplification followed by Illumina-based sequencing from 8 to 12 individual microfilariae from each of the five isolates, obtaining a depth of coverage of approximately 40-75 fold for each. The Illumina sequences were used to create new genome assemblies for all the Wolbachia isolates studied. Comparisons of the Wolbachia sequences revealed more than 3000 sequence variations in each isolate. We identified 67 loci specific in resistant isolates but not in susceptible isolates, including 18 genes affected.Phylogenetic analysis suggested that the endosymbionts of the drug-susceptible isolates are more closely related to each other than to those from any of the resistant parasites. This level of variation in the Wolbachia endosymbionts of D. immitis isolates suggests a potential for selection for resistance against drugs targeting them.

Indexed as

Drug ResistanceGenetic VariationGenome, BacterialAnimalsDirofilaria immitisLactonesMacrocyclic CompoundsWolbachiaLactonesMacrocyclic CompoundsDirofilaria immitisDrug resistanceGenome sequenceWolbachia

Identifiers

PMID32937243
PMCPMC7584739
OpenAlexW3083076913

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.