ArticleThe Journal of cell biology2020
An antibody toolbox to track complex I assembly defines AIF's mitochondrial function.
Article in The Journal of cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- ATP synthase subunits e and g are essential for CaCell death and differentiation · 2026Article
- Mitochondrial Fission Regulator 1-Like Protein Protects the Heart from Ischemia/Reperfusion Injury via Dual Mitochondrial Mechanisms.Research (Washington, D.C.) · 2026Article
- CRABS-ROC, A respirometry protocol for overcoming substrate limitations, reveals excess brain mitochondrial Complex I capacity.Free radical biology & medicine · 2025Article
- Dietary lipid content modifies wah-1/AIFM1-associated phenotypes via LRK-1 and DRP-1 expression in C. elegans.Nature communications · 2025Article
- Calpain2-mediated downregulation of apoptosis-inducing factors impairs mitochondrial function in noise-induced spiral ganglion neuron degeneration.IBRO neuroscience reports · 2025Article
- Article
- Phospholipids can regulate complex I assembly independent of their role in maintaining mitochondrial membrane integrity.Cell reports · 2023Article
- Reverse electron transfer is activated during aging and contributes to aging and age-related disease.EMBO reports · 2023Article
- Resting mitochondrial complex I fromeLife · 2023Article
- Mitochondrial Neurodegeneration: Lessons fromBiomolecules · 2023Review
- Article
- dTtc1, a conserved tetratricopeptide repeat protein, is required for maturation ofFrontiers in cell and developmental biology · 2023Article
- The membrane domain of respiratory complex I accumulates during muscle aging in Drosophila melanogaster.Scientific reports · 2022Article
- Parkin drives pS65-Ub turnover independently of canonical autophagy in Drosophila.EMBO reports · 2022Article
- Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III ineLife · 2022Article
- AIFM1 is a component of the mitochondrial disulfide relay that drives complex I assembly through efficient import of NDUFS5.The EMBO journal · 2022Article
- CG7630 is the Drosophila melanogaster homolog of the cytochrome c oxidase subunit COX7B.EMBO reports · 2022Article
- IDH2-mediated regulation of the biogenesis of the oxidative phosphorylation system.Science advances · 2022Article
- Loss of Drosophila Clueless differentially affects the mitochondrial proteome compared to loss of Sod2 and Pink1.Frontiers in physiology · 2022Article
- Analyzing the integrity of oxidative phosphorylation complexes inSTAR protocols · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
An ability to comprehensively track the assembly intermediates (AIs) of complex I (CI) biogenesis in Drosophila will enable the characterization of the precise mechanism(s) by which various CI regulators modulate CI assembly. Accordingly, we generated 21 novel antibodies to various mitochondrial proteins and used this resource to characterize the mechanism by which apoptosis-inducing factor (AIF) regulates CI biogenesis by tracking the AI profile observed when AIF expression is impaired. We find that when the AIF-Mia40 translocation complex is disrupted, the part of CI that transfers electrons to ubiquinone is synthesized but fails to progress in the CI biosynthetic pathway. This is associated with a reduction in intramitochondrial accumulation of the Mia40 substrate, MIC19. Importantly, knockdown of either MIC19 or MIC60, components of the mitochondrial contact site and cristae organizing system (MICOS), fully recapitulates the AI profile observed when AIF is inhibited. Thus, AIF's effect on CI assembly is principally due to compromised intramitochondrial transport of the MICOS complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.