Evidence map›Paper›PMID 32936529›Full record

ArticleCurrent protocols in cytometry2020

Small Particle Fluorescence and Light Scatter Calibration Using FCM

Joshua A Welsh, Jennifer C Jones

Abstract read
In one paragraph

Article in Current protocols in cytometry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Trial
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  8. Loading of therapeutic cell penetrating peptides into extracellular vesicles for pulmonary fibrosis.Journal of controlled release : official journal of the Controlled Release Society · 2026
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  13. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Joshua A WelshLaboratory of Pathology, Translational Nanobiology Section, Center for Cancer Research, National Institute of Health, National Cancer Institute, Bethesda, Maryland.
Jennifer C JonesLaboratory of Pathology, Translational Nanobiology Section, Center for Cancer Research, National Institute of Health, National Cancer Institute, Bethesda, Maryland.

Funding

Development and use of nanoFACS for analysis and sorting of nanoparticlesZIABC011502 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI JONES, JENNIFER · 2013 to 2025
$8.6M
Novel Combinations of Radiation and ImmunotherapyZIABC011503 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI JONES, JENNIFER · 2013 to 2025
$5.3M
Effect of methodological and biological variability on molecular profiling of extracellular vesicles in cancer detectionR01CA218500 · NCI · NORTHEASTERN UNIVERSITY · PI GHIRAN, IONITA CALIN, IVANOV, ALEXANDER R. · 2018 to 2022
$4.0M
Impact of circadian rhythm in obtaining reference profiles of exRNAs in healthy iU01HL126497 · NHLBI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI GHIRAN, IONITA CALIN · 2014 to 2018
$3.3M
Integrative, multi-parametric characterization of the EV surface protein and nucleic acid landscape by nano-flow and sorting cytometryUG3TR002881 · NCATS · BETH ISRAEL DEACONESS MEDICAL CENTER · PI GHIRAN, IONITA CALIN, JONES, JENNIFER · 2019 to 2020
$816k
Intramural NIH HHS ZIA BC011502Intramural NIH HHS ZIA BC011503NCATS NIH HHS UG3 TR002881NCI NIH HHS R01 CA218500NHLBI NIH HHS U01 HL126497NIH HHS U01 HL126497NIH HHS UG3 TR002881
6 · The paper itself

Abstract

Use of flow cytometry to analyze small particles has been implemented for several decades. More recently, small particle analysis has become increasingly utilized owing to the increased sensitivity of conventional and commercially available flow cytometers along with growing interest in small particles such as extracellular vesicles (EVs). Despite an increase in small particle flow cytometry utilization, a lack of standardization in data reporting has resulted in a growing body of literature regarding EVs that cannot be easily interpreted, validated, or reproduced. Methods for fluorescence and light scatter standardization are well established, and the reagents to perform these analyses are commercially available. Here, we describe FCM

Indexed as

Particle SizeScattering, RadiationSoftwareCalibrationDatabases as TopicFlow CytometryFluorescencecalibrationextracellular vesiclesfluorescencelight scatterstandardization

Identifiers

PMID32936529
PMCPMC8623744

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.