ArticleMolecular plant pathology2020
Identification of Avramr1 from Phytophthora infestans using long read and cDNA pathogen-enrichment sequencing (PenSeq).
Article in Molecular plant pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Diverse haplotypes at a complex Solanum americanum locus confer resistance to Phytophthora infestans and P. capsici.The Plant cell · 2026Article
- Development and application of aFrontiers in plant science · 2025Article
- Conserved effector families render Phytophthora species vulnerable to recognition by NLR receptors in nonhost plants.Nature communications · 2024Article
- Evolution of Phytophthora infestans on its potato host since the Irish potato famine.Nature communications · 2024Article
- Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors.Nature genetics · 2023Article
- Effector-dependent activation and oligomerization of plant NRC class helper NLRs by sensor NLR immune receptors Rpi-amr3 and Rpi-amr1.The EMBO journal · 2023Article
- How do emerging long-read sequencing technologies function in transforming the plant pathology research landscape?Plant molecular biology · 2022Review
- Thirty years of resistance: Zig-zag through the plant immune system.The Plant cell · 2022Review
- Phytophthora infestans RXLR effector Pi04089 perturbs diverse defense-related genes to suppress host immunity.BMC plant biology · 2021Article
- Allelic variants of the NLR protein Rpi-chc1 differentially recognize members of the Phytophthora infestans PexRD12/31 effector superfamily through the leucine-rich repeat domain.The Plant journal : for cell and molecular biology · 2021Article
- Comparative Analysis of Host-Associated Variation inFrontiers in microbiology · 2021Article
- Structural specificity in plant-filamentous pathogen interactions.Molecular plant pathology · 2020Review
- Identification of Avramr1 from Phytophthora infestans using long read and cDNA pathogen-enrichment sequencing (PenSeq).Molecular plant pathology · 2020Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Potato late blight, caused by the oomycete pathogen Phytophthora infestans, significantly hampers potato production. Recently, a new Resistance to Phytophthora infestans (Rpi) gene, Rpi-amr1, was cloned from a wild Solanum species, Solanum americanum. Identification of the corresponding recognized effector (Avirulence or Avr) genes from P. infestans is key to elucidating their naturally occurring sequence variation, which in turn informs the potential durability of the cognate late blight resistance. To identify the P. infestans effector recognized by Rpi-amr1, we screened available RXLR effector libraries and used long read and cDNA pathogen-enrichment sequencing (PenSeq) on four P. infestans isolates to explore the untested effectors. Using single-molecule real-time sequencing (SMRT) and cDNA PenSeq, we identified 47 highly expressed effectors from P. infestans, including PITG_07569, which triggers a highly specific cell death response when transiently coexpressed with Rpi-amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1. Here we demonstrate that long read and cDNA PenSeq enables the identification of full-length RXLR effector families and their expression profile. This study has revealed key insights into the evolution and polymorphism of a complex RXLR effector family that is associated with the recognition by Rpi-amr1.
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