Evidence map›Paper›PMID 32935195›Full record

ArticleJournal of fluorescence2020

The Effects of Different Fluorescent Indicators in Observing the Changes of the Mitochondrial Membrane Potential during Oxidative Stress-Induced Mitochondrial Injury of Cardiac H9c2 Cells.

Yahan Sun, Kunyan Zhou, Mali He, Ying Gao, Danjie Zhang, Yanwen Bai, Yuezhao Lai, Mengying Liu, Xuechao Han, Sen Xu and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of fluorescence, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Yahan SunHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Kunyan ZhouDepartment of Cardiovascular, Suning Renmin Hospital, Cangzhou, 061000, Hebei Province, China.
Mali HeHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Ying GaoHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Danjie ZhangHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Yanwen BaiHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Yuezhao LaiHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Mengying LiuHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Xuechao HanHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Sen XuHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Wei TianHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Jingman XuHeart Institute, School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China. xujingman1983@163.com.ORCID http://orcid.org/0000-0001-8552-4134
North China University of Science and Technology · CNSuning (China) · CN

Funding

Department of Education of Hebei Province ZD2017063Hebei Provincial university student innovation project X2018022Natural Science Foundation of Hebei Province H2018209378Young Scientists Fund 81700324
6 · The paper itself

Abstract

We evaluated the ability of different fluorescent indicators by various analytical instruments, including a laser scanning confocal microscope (LSCM), fluorescence plate reader, and flow cytometer (FCM), to measure the mitochondrial membrane potential (ΔΨm) of cardiac H9c2 cells during oxidative stress-induced mitochondrial injury. The mitochondrial oxygen consumption rate and a transmission electron microscope were used to detect changes in mitochondrial functions and morphology, respectively. Cardiac H9c2 cells were exposed to H

Indexed as

Membrane Potential, MitochondrialOxidative StressAnimalsCell LineFluorescent DyesMitochondria, HeartRatsReactive Oxygen SpeciesFluorescent DyesReactive Oxygen SpeciesDynamic measurementsEndpoint measurementsFluorescence plate readerLaser scanning confocal microscopeMitochondrial membrane potential

Identifiers

PMID32935195
OpenAlexW3085010079

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.