Evidence map›Paper›PMID 32935194›Full record

ArticleBiomolecular NMR assignments2021

Erin Skeens, Kyle W East, George P Lisi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecular NMR assignments, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Erin SkeensDepartment of Molecular Biology, Cellular Biology & Biochemistry, Brown University, Providence, RI, 02903, USA.ORCID http://orcid.org/0000-0002-0580-1291
Kyle W EastDepartment of Molecular Biology, Cellular Biology & Biochemistry, Brown University, Providence, RI, 02903, USA.
George P LisiDepartment of Molecular Biology, Cellular Biology & Biochemistry, Brown University, Providence, RI, 02903, USA. george_lisi@brown.edu.ORCID http://orcid.org/0000-0001-8878-5655
Brown University · US

Funding

The role of Sirtuins in neurodegenerative diseaseP20GM109035 · NIGMS · BROWN UNIVERSITY · PI WONG, IAN Y · 2016 to 2025
$22.6M
Studies of Allostery between Multi-domain Proteins and Nucleic Acid ComplexesR01GM136815 · NIGMS · YALE UNIVERSITY · PI BATISTA, VICTOR S · 2021 to 2024
$1.4M
NIGMS NIH HHS P20 GM109035
6 · The paper itself

Abstract

Rec3 is a subdomain of the recognition (Rec) lobe within CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-associated protein Cas9 that is involved in nucleic acid binding and is critical to HNH endonuclease activation. Here, we report the backbone resonance assignments of an engineered construct of the Rec3 subdomain from Streptococcus pyogenes Cas9. We also analyze backbone chemical shift data to predict secondary structure and an overall fold that is consistent with that of Rec3 from the full-length S. pyogenes Cas9 protein.

Indexed as

Nuclear Magnetic Resonance, BiomolecularProtein DomainsStreptococcus pyogenesProtein Structure, SecondaryCas9CRISPRNMR assignmentsRec3Recognition lobe

Identifiers

PMID32935194
PMCPMC8635283
OpenAlexW3087360193

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.