ArticleNature communications2020
Light microscopy based approach for mapping connectivity with molecular specificity.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed.
- Simultaneous brain-wide single-cell recording resolves spatiotemporal memory architecture.bioRxiv : the preprint server for biology · 2026Article
- Long-range axon branching: contributions to brain network plasticity and repair.Nature reviews. Neuroscience · 2026Review
- 3D Expansion-PALM (PhotoActivated Localization Microscopy) Dissects Protein-Protein Interactions Down to the Molecular Scale in Bacteria.Microorganisms · 2026Article
- Mitochondria-associated membranes in heart failure: from molecular mechanisms to therapeutic targets.Frontiers in physiology · 2026Review
- µCodes: A Universal Grid Platform for Microscale Mapping, Microscopy Navigation, and Multimodal Imaging.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context.bioRxiv : the preprint server for biology · 2025Article
- Review
- A parallelly distributed microscope and software system for scalable high-throughput multispectral 3D imaging.bioRxiv : the preprint server for biology · 2025Article
- Article
- Comparative prospects of imaging methods for whole-brain mammalian connectomics.Cell reports methods · 2025Review
- Dense, continuous membrane labeling and expansion microscopy visualization of ultrastructure in tissues.Nature communications · 2025Article
- Data-driven synapse classification reveals a logic of glutamate receptor diversity.bioRxiv : the preprint server for biology · 2025Article
- Super-resolution imaging of the neuronal cytoskeleton.Npj imaging · 2024Review
- Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain.Nature communications · 2024Article
- Advances in microscopy characterization techniques for lipid nanocarriers in drug delivery: a comprehensive review.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Adaptation of Magnified Analysis of the Proteome for Excitatory Synaptic Proteins in Varied Samples and Evaluation of Cell Type-Specific Distributions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues.bioRxiv : the preprint server for biology · 2024Article
- Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding.Science translational medicine · 2024Article
- Purkinje cell microzones mediate distinct kinematics of a single movement.Nature communications · 2023Article
- 3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy.Communications biology · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Mapping neuroanatomy is a foundational goal towards understanding brain function. Electron microscopy (EM) has been the gold standard for connectivity analysis because nanoscale resolution is necessary to unambiguously resolve synapses. However, molecular information that specifies cell types is often lost in EM reconstructions. To address this, we devise a light microscopy approach for connectivity analysis of defined cell types called spectral connectomics. We combine multicolor labeling (Brainbow) of neurons with multi-round immunostaining Expansion Microscopy (miriEx) to simultaneously interrogate morphology, molecular markers, and connectivity in the same brain section. We apply this strategy to directly link inhibitory neuron cell types with their morphologies. Furthermore, we show that correlative Brainbow and endogenous synaptic machinery immunostaining can define putative synaptic connections between neurons, as well as map putative inhibitory and excitatory inputs. We envision that spectral connectomics can be applied routinely in neurobiology labs to gain insights into normal and pathophysiological neuroanatomy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.