ArticleMicrobial cell factories2020
Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 26 citations in OpenAlex.
- Biochemical characterization of chitinolytic Bacillus isolates from a hypersaline wetland and their biopriming potential for improving wheat germination under salinity stress.BMC plant biology · 2026Article
- Efficacy of chitinases from mangrove wetland derivedFrontiers in microbiology · 2026Article
- High-level expression of zearalenone lactonohydrolase ZenG in Pichia pastoris via combinatorial strategy.Archives of microbiology · 2025Article
- Recombinant expression and characterization of the endochitinase Chit36-TA from Trichoderma asperellum in Komagataella phaffii for chitin degradation of black soldier fly exuviae.Bioprocess and biosystems engineering · 2024Article
- Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-910.Bioresources and bioprocessing · 2024Article
- Biochemical Properties of a Cold-Active Chitinase from MarineMarine drugs · 2023Article
- Recombinant expression of hen egg white lysozyme with the assistance of xylanase fusion partner inBioengineered · 2022Article
- High-Level Extracellular Expression of a New β-N-Acetylglucosaminidase inFrontiers in microbiology · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacillus licheniformis and co-expression of molecular chaperon HAC1 was constructed. The amount of recombinant ChiA in the supernatant of high-cell-density fermentation reaches a maximum of 12.7 mg/mL, which is 24-fold higher than that reported in the previous study. The recombinant ChiA can hydrolyze 30% collodidal chitin with 74% conversion ratio, and GlcNAc is the most abundant hydrolysis product, followed by N, N'-diacetylchitobiose. Combined with BsNagZ, the hydrolysate of ChiA can be further transformed into GlcNAc with 88% conversion ratio. Additionally, the hydrolysate of ChiA can obviously accelerate the germination growth of rice and wheat, increasing the seedling height and root length by at least 1.6 folds within 10 days.
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