ArticleBMC veterinary research2020
Effects of long-distance transportation on blood constituents and composition of the nasal microbiota in healthy donkeys.
Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 12 citations in OpenAlex.
- Establishment and evaluation of hematological and serum biochemical reference intervals in Chinese local donkey populations.BMC veterinary research · 2026Article
- Associations Between Fine Particulate Matter-Associated Bacteria and Respiratory Tract Microbiota in Pigs.Animals : an open access journal from MDPI · 2026Article
- Advances in Donkey Disease Surveillance and Microbiome Characterization in China.Microorganisms · 2025Review
- Gut microbiome regulation in equine animals: current understanding and future perspectives.Frontiers in microbiology · 2025Review
- Multi-omics investigation into long-distance road transportation effects on respiratory health and immunometabolic responses in calves.Microbiome · 2024Article
- A review of genetic resources and trends of omics applications in donkey research: focus on China.Frontiers in veterinary science · 2024Review
- Increased freedom of head movement mitigates stress and bacterial load in the airways of horses during transport.Frontiers in veterinary science · 2024Article
- Auction market placement and a rest stop during transportation affect the respiratory bacterial microbiota of beef cattle.Frontiers in microbiology · 2023Article
- Serum Cortisol and Its Correlation with Leucocyte Profile and Circulating Lipids in Donkeys (Animals : an open access journal from MDPI · 2022Article
- Nasopharyngeal Microbiomes in Donkeys SheddingFrontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundThis study aims to determine the effects of transportation on the nasal microbiota of healthy donkeys using 16S rRNA sequencing.
resultsDeep nasal swabs and blood were sampled from 14 donkeys before and after 21 hours' long-distance transportation. The values of the plasma hormone (cortisol (Cor), adrenocorticotrophic hormone (ACTH)), biochemical indicators (total protein (TP), albumin (ALB), creatinine (CREA), lactic dehydrogenase (LDH), aspartate transaminase (AST), creatine kinase (CK), blood urea (UREA), plasma glucose (GLU)) and blood routine indices (white blood cell (WBC), lymphocyte (LYM), neutrophil (NEU), red blood cell (RBC), hemoglobin (HGB)) were measured. 16S rRNA sequencing was used to assess the nasal microbiota, including alpha diversity, beta diversity, and phylogenetic structures. Results showed that levels of Cor, ACTH, and heat-shock protein 90 (HSP90) were significantly increased (p < 0.05) after long-distance transportation. Several biochemical indicators (AST, CK) and blood routine indices (Neu, RBC, and HGB) increased markedly (p < 0.05), but the LYM decreased significantly (p < 0.05). Nine families and eight genera had a mean relative abundance over 1%. The predominant phyla in nasal microbiota after and before transportation were Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Transportation stress induced significant changes in terms of nasal microbiota structure compared with those before transportation based on principal coordinate analysis (PCoA) coupled with analysis of similarities (ANOSIM) (p < 0.05). Among these changes, a notably gain in Proteobacteria and loss in Firmicutes at the phylum level was observed.
conclusionsThese results suggest transportation can cause stress to donkeys and change the richness and diversity of nasal microbiota. Further studies are required to understand the potential effect of these microbiota changes on the development of donkey respiratory diseases.
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