Evidence map›Paper›PMID 32931871›Full record

ArticleBrain, behavior, and immunity2021

Best practice recommendations for the measurement and interpretation of salivary proinflammatory cytokines in biobehavioral research.

Jenna L Riis, Hedyeh Ahmadi, Katrina R Hamilton, Tracey Hand, Douglas A Granger

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain, behavior, and immunity, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jenna L RiisInstitute for Interdisciplinary Salivary Bioscience Research, University of California, Irvine, CA, USA; Department of Psychological Science, University of California, Irvine, CA, USA. Electronic address: jriis@uci.edu.
Hedyeh AhmadiInstitute for Interdisciplinary Salivary Bioscience Research, University of California, Irvine, CA, USA.
Katrina R HamiltonInstitute for Interdisciplinary Salivary Bioscience Research, University of California, Irvine, CA, USA.
Tracey HandCenter for Interdisciplinary Salivary Bioscience Research, Johns Hopkins University School of Nursing, Baltimore, MD, USA.
Douglas A GrangerInstitute for Interdisciplinary Salivary Bioscience Research, University of California, Irvine, CA, USA; Department of Psychological Science, University of California, Irvine, CA, USA; Department of Acute and Chronic Care, Johns Hopkins University School of Nursing, Baltimore, MD, USA; Department of Pediatrics Johns Hopkins University School of Medicine, Baltimore, MD, USA; Salivary Bioscience Laboratory and Department of Psychology, University of Nebraska, Lincoln, NE, USA.
University of California, Irvine · USJohns Hopkins University · US

Funding

Early Life Stress and the Environmental Origins of Disease: a Population-based Prospective Longitudinal Study of Children in Rural Poverty (ECHO)UH3OD023332 · OD · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI KOPP, LISA MICHELLE, SWINGLER, MARGARET M. · 2018 to 2022
$22.7M
NICHD NIH HHS HHSN267200700048CNIH HHS UH3 OD023332
6 · The paper itself

Abstract

Despite the integration of salivary inflammatory cytokines into research across the biobehavioral, psychological, clinical, and health-related disciplines, there is little guidance regarding the biospecimen collection, handling, and storage practices that maximize the quality and validity of salivary cytokine data. Furthermore, associations between salivary cytokines and measures related to oral health are rarely assessed and accounted for in studies outside the oral health fields. To address these gaps, we examine the sensitivity of salivary interleukin-1β (IL-1β), IL-6, IL-8, and tumor necrosis factor-α (TNF-α) to changes in saliva sample collection technique and cold chain management procedures. Using subsets of saliva samples collected from 150 healthy adults, we measure salivary IL-1β, IL-6, IL-8, TNF-α, and other oral health-related indices (i.e., blood contamination [transferrin], and salivary matrixmallotprotienase-8). In addition to examining changes in cytokine levels associated with sample collection technique and cold chain management procedures, we assess relations between cytokine concentrations and levels of other oral health-related measures. We found that IL-1β, IL-6, and IL-8 were more robust to changes in sample collection and cold chain management procedures than TNF-α, and all cytokines were positively associated with other oral health-related measures. Based on our findings, we recommend analyte-specific guidance for measuring and interpreting salivary cytokine concentrations.

Indexed as

CytokinesSalivaAdultHumansInterleukin-1betaTumor Necrosis Factor-alphaCytokinesInterleukin-1betaTumor Necrosis Factor-alphaBest practicesCold chainCytokineInflammationSaliva

Identifiers

PMID32931871
PMCPMC8164445
OpenAlexW3085491747

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.