Evidence map›Paper›PMID 32930932›Full record

ArticleMolecular biology reports2020

First de novo genome specific development, characterization and validation of simple sequence repeat (SSR) markers in Genus Salvadora.

Maneesh S Bhandari, Rajendra K Meena, Arzoo Shamoon, Shanti Saroj, Rama Kant, Shailesh Pandey

Abstract readValidation Study
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. De novo SSR markers development and genetic diversity assessment inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2025
    Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Genome skimming-based simple sequence repeat (SSR) marker discovery and characterization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021
    Article
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Maneesh S BhandariDivision of Genetics & Tree Improvement, Forest Research Institute, Dehradun, Uttarakhand, 248 195, India. bhandarims@icfre.org.ORCID http://orcid.org/0000-0002-7069-7048
Rajendra K MeenaDivision of Genetics & Tree Improvement, Forest Research Institute, Dehradun, Uttarakhand, 248 195, India.ORCID http://orcid.org/0000-0001-8545-7349
Arzoo ShamoonDivision of Genetics & Tree Improvement, Forest Research Institute, Dehradun, Uttarakhand, 248 195, India.ORCID http://orcid.org/0000-0001-6908-9670
Shanti SarojDivision of Genetics & Tree Improvement, Forest Research Institute, Dehradun, Uttarakhand, 248 195, India.
Rama KantDivision of Genetics & Tree Improvement, Forest Research Institute, Dehradun, Uttarakhand, 248 195, India.
Shailesh PandeyForest Pathology Discipline, Division of Forest Protection, Forest Research Institute, Dehradun, Uttarakhand, 248 006, India.ORCID http://orcid.org/0000-0002-2499-2978

Funding

Ministry of Environment, Forest and Climate change (MoEF&CC), Government of India, New Delhi No. 14/8/2013-RE; dated 09th May, 2016
6 · The paper itself

Abstract

Salvadoraceae constitutes ecologically imperative desert families of 3 genera-Azima, Dobera and Salvadora. Under genus Salvadora of this family, S. oleoides is a keystone species of socio-economic and medicinal value. This species naturally grows in the arid zones but currently experiencing severe fragmentation due to land use change and reduced regeneration, which may have resulted in the depletion of genetic diversity. Hence, it is up-most important to develop genomic resources for studying the population genetics in S. oleoides. This study aims to develop robust microsatellites markers, which were not yet reported in genus Salvodora due to lack of genome sequence information. We developed novel microsatellites markers in S. oleoides using Illumina paired-end sequencing technology. In total, 14,552 simple sequence repeat (SSR) markers were successfully designed from 21,055 microsatellite repeats detected in the 13 Gb raw sequence data. Afterwards, a subset of 101 SSRs were randomly selected and validated, 94 primers were successfully amplified and 34 showed polymorphisms. These SSRs were used to estimate the measures of genetic diversity in three natural populations of state Rajasthan and Gujarat. Importantly, average number of alleles (N

Indexed as

Genome, PlantMicrosatellite RepeatsPolymorphism, GeneticDNA, PlantHigh-Throughput Nucleotide SequencingSalvadoraceaeSpecies SpecificityDNA, PlantGenetic diversityGenome sequencingMicrosatellite markersSalvadoraceaeSalvadora oleoidesSalvadora persica

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.