ArticleAnalytical and bioanalytical chemistry2021
Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
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- Novel Insights into the Distribution and Effects of Perfluorooctanesulfonic acid (PFOS) in the Nervous System of a Frog Tadpole Model by Mass Spectrometry Imaging.Environmental science & technology · 2026Article
- Tissue and extracellular matrix remodeling of the subchondral bone during osteoarthritis of knee joints as revealed by spatial mass spectrometry imaging.Bone research · 2026Article
- Sample Preparation for Multi-Omics Analysis: Considerations and Guidance for Identifying the Ideal Workflow.Proteomics · 2025Review
- Mass Spectrometry Imaging: Revolutionizing Molecular Insights in Infectious Diseases Research.Pathogens (Basel, Switzerland) · 2025Review
- MALDI-MSI: A potential game changer in forensic sciences.Forensic science, medicine, and pathology · 2025Review
- Comprehensive High-Spatial-Resolution Imaging Metabolomics Workflow for Heterogeneous Tissues.Analytical chemistry · 2025Article
- Spatial Distribution of Brain PET Tracers by MALDI Imaging.Journal of the American Society for Mass Spectrometry · 2025Article
- Comprehensive MALDI mass spectrometry imaging of tumor regions post-neoadjuvant therapy.Analytical and bioanalytical chemistry · 2025Article
- Uncovering lipid dynamics in Staphylococcus aureus osteomyelitis using multimodal imaging mass spectrometry.Cell chemical biology · 2024Article
- Applications of MALDI mass spectrometry in forensic science.Analytical and bioanalytical chemistry · 2024Review
- Mass Spectrometry Reveals Molecular Effects of Citrulline Supplementation during Bone Fracture Healing in a Rat Model.Journal of the American Society for Mass Spectrometry · 2024Article
- Article
- Sample Preparation Method for MALDI Mass Spectrometry Imaging of Fresh-Frozen Spines.Analytical chemistry · 2023Article
- High-ResolutionAnalytical chemistry · 2023Article
- Mass spectrometry imaging for biosolids characterization to assess ecological or health risks before reuse.Nature communications · 2023Article
- How to sample a seizure plant: the role of the visualization spatial distribution analysis ofForensic sciences research · 2023Article
- Spatial Lipidomic Profiling of Mouse Joint Tissue Demonstrates the Essential Role of PHOSPHO1 in Growth Plate Homeostasis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples. The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue. Undecalcified sections are preferred to retain sample integrity as much as possible or to study the tissue-bone bio interface in particular. Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively. Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials. The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning. Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue. The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples. Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated. DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode. The limit of detection (LOD) is estimated to approximately 50 pg/spot on bone tissue. The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle. The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios. The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
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Registered trials
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