Evidence map›Paper›PMID 32930298›Full record

ArticleAnalytical methods : advancing methods and applications2020

Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules.

Fatemeh Shafiei, Kathleen McAuliffe, Yousef Bagheri, Zhining Sun, Qikun Yu, Rigumula Wu, Mingxu You

Open access · greenAbstract read
In one paragraph

Article in Analytical methods : advancing methods and applications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Recent Advances in Aptamer Sensors.Sensors (Basel, Switzerland) · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Fatemeh ShafieiDepartment of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. rigumulawu@umass.edu mingxuyou@umass.edu.
Kathleen McAuliffeDepartment of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. rigumulawu@umass.edu mingxuyou@umass.edu.
Yousef BagheriDepartment of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. rigumulawu@umass.edu mingxuyou@umass.edu.
Zhining SunDepartment of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. rigumulawu@umass.edu mingxuyou@umass.edu.
Qikun YuDepartment of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. rigumulawu@umass.edu mingxuyou@umass.edu.
Rigumula WuDepartment of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. rigumulawu@umass.edu mingxuyou@umass.edu.
Mingxu YouDepartment of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA. rigumulawu@umass.edu mingxuyou@umass.edu.ORCID 0000-0002-5279-2340
Amherst College · US

Funding

Genetically-encoded fluorescent RNA sensors for measuring transport of antibiotics into the cytoplasm of Gram-negative pathogens and development of efflux pump inhibitorsR01AI136789 · NIAID · HARVARD MEDICAL SCHOOL · PI LORY, STEPHEN · 2018 to 2022
$3.6M
NIAID NIH HHS R01 AI136789
6 · The paper itself

Abstract

Sensors based on fluorogenic RNA aptamers have emerged in recent years. These sensors have been used for in vitro and intracellular detection of a broad range of biological and medical targets. However, the potential application of fluorogenic RNA-based sensors for point-of-care testing is still little studied. Here, we report a paper substrate-based portable fluorogenic RNA sensor system. Target detection can be simply performed by rehydration of RNA sensor-embedded filter papers. This affordable sensor system can be used for the selective, sensitive, and rapid detection of different target analytes, such as antibiotics and cellular signaling molecules. We believe that these paper-based fluorogenic RNA sensors show great potential for point-of-care testing of a wide range of targets from small molecules, nucleic acids, proteins, to various pathogens.

Indexed as

Aptamers, NucleotideBiosensing TechniquesAnti-Bacterial AgentsProteinsRNAAnti-Bacterial AgentsAptamers, NucleotideProteinsRNA

Identifiers

PMID32930298
PMCPMC7747226
OpenAlexW3028297273

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.