Evidence map›Paper›PMID 32929632›Full record

ArticleParasitology research2020

First molecular characterization of Sarcocystis miescheriana in wild boars (Sus scrofa) from Latvia.

Petras Prakas, Viktorija Kirillova, Anna Dzerkale, Muza Kirjušina, Dalius Butkauskas, Inese Gavarāne, Eglė Rudaitytė-Lukošienė, Gediminas Šulinskas

Abstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. International journal for parasitology. Parasites and wildlife · 2025
    Article
  4. First molecular characterisation ofJournal of veterinary research · 2024
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Molecular detection of Toxoplasma gondii, Neospora caninum and Sarcocystis spp in tissues of Sus scrofa slaughtered in southern Brazil.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2023
    Article
  10. Molecular Identification of ProtozoanLife (Basel, Switzerland) · 2022
    Article
  11. A Review onAnimals : an open access journal from MDPI · 2022
    Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Petras PrakasNature Research Centre, Akademijos 2, LT-08412, Vilnius, Lithuania. prakaspetras@gmail.com.ORCID http://orcid.org/0000-0002-5115-2659
Viktorija KirillovaInstitute of Life Sciences and Technology, Daugavpils University, Parādes 1A, Daugavpils, LV-5401, Latvia.
Anna DzerkaleInstitute of Life Sciences and Technology, Daugavpils University, Parādes 1A, Daugavpils, LV-5401, Latvia.
Muza KirjušinaInstitute of Life Sciences and Technology, Daugavpils University, Parādes 1A, Daugavpils, LV-5401, Latvia.
Dalius ButkauskasNature Research Centre, Akademijos 2, LT-08412, Vilnius, Lithuania.
Inese GavarāneInstitute of Life Sciences and Technology, Daugavpils University, Parādes 1A, Daugavpils, LV-5401, Latvia.
Eglė Rudaitytė-LukošienėNature Research Centre, Akademijos 2, LT-08412, Vilnius, Lithuania.
Gediminas ŠulinskasNature Research Centre, Akademijos 2, LT-08412, Vilnius, Lithuania.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Various muscle samples of wild boar (Sus scrofa) from Latvia were studied for the presence of Sarcocystis infection by means of morphological and molecular methods. Sarcocysts were detected in 122 out of 140 (87.1%) wild boar examined. According to the morphological appearance of sarcocysts, the observed cysts belonged to one morphological type and resembled Sarcocystis miescheriana. Twenty-three sarcocysts isolated from the muscles of Latvian wild boars were molecularly characterized at 18S rRNA, ITS1 and cox1. Additionally, eight sarcocysts obtained from Lithuanian wild boars were subjected to molecular analysis in order to compare intraspecific genetic variability. The amplified 18S rRNA region using newly designed primers is sufficiently variable to separate S. miecheriana from S. suihominis. All Latvian and Lithuanian isolates were confirmed belonging to S. miescheriana. No genetic variation was detected within 18S rRNA and ITS1. By contrast, the high intraspecific genetic variability of S. miescheriana was observed within cox1 since each newly obtained sequence represented a unique haplotype. The comparison made using S. miescheriana isolates from Italian and Japanese wild boar and Chinese domestic pig revealed the genetic similarity of the samples depending on their geographical distances. The current study provides the first detection of Sarcocystis infection in wild boars from Latvia and molecular characterization of S. miescheriana.

Indexed as

AnimalsDNA, ProtozoanHaplotypesLatviaMusclesPhylogenyPolymerase Chain ReactionRNA, Ribosomal, 18SSarcocystisSarcocystosisSpecies SpecificitySus scrofaSwineDNA, ProtozoanRNA, Ribosomal, 18Scox1Population geneticsSarcocystis miescherianaWild boar

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.