ArticleThe ISME journal2021
Proteome specialization of anaerobic fungi during ruminal degradation of recalcitrant plant fiber.
Article in The ISME journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 94 citations in OpenAlex.
- Nutritional Strategies for Methane, Nitrogen, and Phosphorus Mitigation in Ruminants: Mechanisms, Applications, and Regional Adaptations.Microorganisms · 2026Review
- Diversity and community structure of anaerobic gut fungi in camels.bioRxiv : the preprint server for biology · 2026Article
- The Dairy Ruminant Gut Microbiome: Profile, Responsiveness to Seasonality and Impact on Milk Quality.Environmental microbiology · 2026Review
- Rumen Mycobiome Thiamine Metabolism Contributes to Subacute Rumen Acidosis Tolerance in Goats Through Enhancing Epithelial Cell Proliferation via IGFBP2/IGF1 Axis Activation.Exploration (Beijing, China) · 2026Article
- Integrative analysis of rumen microbiota and host multi-organ interactions underlying feed conversion efficiency in Hu sheep.Journal of animal science and biotechnology · 2026Article
- The developmental trajectory and maturation of the Hulunbuir sheep (Ovis aries) microbiome.Animal microbiome · 2026Article
- Metagenomic and metabolomic analyses of rumen fiber digestion in Mongolian cattle fed fresh grass versus hay.Microbiology spectrum · 2026Article
- Carbohydrate-active enzyme architectures reflect distinct fungal and bacterial strategies for lignocellulose conversion to volatile fatty acids.ISME communications · 2026Article
- Red seaweed supplementation suppresses methanogenesis in the rumen, revealing potentially advantageous traits among hydrogenotrophic bacteria.Microbiome · 2025Article
- Anaerobic fungi: effective warriors in lignocellulosic biomass degradation and fermentation.FEMS microbiology ecology · 2025Review
- Article
- The crosstalk between host and rumen microbiome in cattle: insights from multi-omics approaches and genome-wide association studies.World journal of microbiology & biotechnology · 2025Review
- Cellulase enhancing rumen microbiome of Tan sheep indicates plastic responses to seasonal variations of diet in the typical steppe.BMC microbiology · 2025Article
- Probing the eukaryotic microbes of ruminants with a deep-learning classifier and comprehensive protein databases.Genome research · 2025Article
- Studies on the concerted interaction of microbes in the gastrointestinal tract of ruminants on lignocellulose and its degradation mechanism.Frontiers in microbiology · 2025Review
- Multi-omics analysis provides insights into mechanisms of intestinal fungi adaptation to dietary carbohydrates.Current research in microbial sciences · 2025Article
- Lignocellulose degradation in bacteria and fungi: cellulosomes and industrial relevance.Frontiers in microbiology · 2025Review
- Multiomics of yaks reveals significant contribution of microbiome into host metabolism.NPJ biofilms and microbiomes · 2024Article
- Seasonal stability of the rumen microbiome contributes to the adaptation patterns to extreme environmental conditions in grazing yak and cattle.BMC biology · 2024Article
- Biochemical characterization of a SusD-like protein involved in β-1,3-glucan utilization by an uncultured cow rumenmSphere · 2024Article
Corrections and comments
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Authors and funding
20 authors at 8 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rumen harbors a complex microbial mixture of archaea, bacteria, protozoa, and fungi that efficiently breakdown plant biomass and its complex dietary carbohydrates into soluble sugars that can be fermented and subsequently converted into metabolites and nutrients utilized by the host animal. While rumen bacterial populations have been well documented, only a fraction of the rumen eukarya are taxonomically and functionally characterized, despite the recognition that they contribute to the cellulolytic phenotype of the rumen microbiota. To investigate how anaerobic fungi actively engage in digestion of recalcitrant fiber that is resistant to degradation, we resolved genome-centric metaproteome and metatranscriptome datasets generated from switchgrass samples incubated for 48 h in nylon bags within the rumen of cannulated dairy cows. Across a gene catalog covering anaerobic rumen bacteria, fungi and viruses, a significant portion of the detected proteins originated from fungal populations. Intriguingly, the carbohydrate-active enzyme (CAZyme) profile suggested a domain-specific functional specialization, with bacterial populations primarily engaged in the degradation of hemicelluloses, whereas fungi were inferred to target recalcitrant cellulose structures via the detection of a number of endo- and exo-acting enzymes belonging to the glycoside hydrolase (GH) family 5, 6, 8, and 48. Notably, members of the GH48 family were amongst the highest abundant CAZymes and detected representatives from this family also included dockerin domains that are associated with fungal cellulosomes. A eukaryote-selected metatranscriptome further reinforced the contribution of uncultured fungi in the ruminal degradation of recalcitrant fibers. These findings elucidate the intricate networks of in situ recalcitrant fiber deconstruction, and importantly, suggest that the anaerobic rumen fungi contribute a specific set of CAZymes that complement the enzyme repertoire provided by the specialized plant cell wall degrading rumen bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.