ArticleNature plants2020
Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli.
Article in Nature plants, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- Machine learning-assisted directed evolution of plant Rubisco.Science advances · 2026Article
- Translating functional molecular knowledge into crop-breeding success.Nature reviews. Genetics · 2026Review
- Rewinding evolution in planta: A Rubisco-null platform validates high-performance ancestral enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Reprogramming encapsulins into modular carbon-fixing nanocompartments.Nature communications · 2025Article
- Directed evolution of a plant Rubisco chaperone with altered client recognition.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity.Nature plants · 2025Article
- Replacement of large subunit N terminus enabled biogenesis of different plant Rubiscos in E. coli.Plant biotechnology journal · 2025Article
- A SynBio explosion: a whole new world for Rubisco engineering.Journal of experimental botany · 2025Article
- Review
- Genetic engineering of RuBisCO by multiplex CRISPR editing small subunits in rice.Plant biotechnology journal · 2025Article
- Increasing Rubisco as a simple means to enhance photosynthesis and productivity now without lowering nitrogen use efficiency.The New phytologist · 2025Review
- Towards engineering a hybrid carboxysome.Photosynthesis research · 2023Article
- A Bacterial Form I' Rubisco Has a Smaller Carbon Isotope Fractionation than Its Form I Counterpart.Biomolecules · 2023Article
- Chitosan-Modified Polyethyleneimine Nanoparticles for Enhancing the Carboxylation Reaction and Plants' COACS nano · 2023Article
- Producing fast and active Rubisco in tobacco to enhance photosynthesis.The Plant cell · 2023Article
- Temperature-induced changes in Arabidopsis Rubisco activity and isoform expression.Journal of experimental botany · 2023Article
- Red Rubiscos and opportunities for engineering green plants.Journal of experimental botany · 2023Review
- The small subunit of Rubisco and its potential as an engineering target.Journal of experimental botany · 2023Review
- Correlative adaptation between Rubisco and CONature plants · 2022Article
- Improving the efficiency of Rubisco by resurrecting its ancestors in the family Solanaceae.Science advances · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) catalyses the first step in carbon fixation and is a strategic target for improving photosynthetic efficiency. In plants, Rubisco is composed of eight large and eight small subunits, and its biogenesis requires multiple chaperones. Here, we optimized a system to produce tobacco Rubisco in Escherichia coli by coexpressing chaperones in autoinduction medium. We successfully assembled tobacco Rubisco in E. coli with each small subunit that is normally encoded by the nuclear genome. Even though each enzyme carries only a single type of small subunit in E. coli, the enzymes exhibit carboxylation kinetics that are very similar to the carboxylation kinetics of the native Rubisco. Tobacco Rubisco assembled with a recently discovered trichome small subunit has a higher catalytic rate and a lower CO
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.