Evidence map›Paper›PMID 32929160›Full record

ArticleScientific reports2020

Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis.

Amanda R Burnham-Marusich, Ryan K Olsen, Jacqueline Scarbrough, Alexander Kvam, Wei Yang, Lindsey Zimmerman, James J Dunn, Tod Merkel, Thomas R Kozel

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Amanda R Burnham-MarusichDxDiscovery, Reno, NV, USA. burnham-marusich@dxdiscovery.com.
Ryan K OlsenDxDiscovery, Reno, NV, USA.
Jacqueline ScarbroughDxDiscovery, Reno, NV, USA.
Alexander KvamDepartment of Microbiology and Immunology, University of Nevada, Reno School of Medicine, Reno, NV, USA.
Wei YangDepartment of Community Health Sciences, University of Nevada, Reno, Reno, NV, USA.
Lindsey ZimmermanCenter for Biological Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
James J DunnTexas Children's Hospital, Houston, TX, USA.
Tod MerkelCenter for Biological Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
Thomas R KozelDxDiscovery, Reno, NV, USA.
DxDiscovery (United States) · USUniversity of Nevada, Reno · USCenter for Drug Evaluation and Research · USTexas Children's Hospital · US

Funding

Point-of-care lateral flow immunoassay for diagnosis of pertussisR44AI109891 · NIAID · DXDISCOVERY, INC. · PI BURNHAM-MARUSICH, AMANDA · 2016 to 2025
$4.0M
Point-of-care immunoassay for early diagnosis of pertussisR43AI109891 · NIAID · DXDISCOVERY, INC. · PI BURNHAM-MARUSICH, AMANDA, KOZEL, THOMAS R · 2014 to 2015
$590k
NIAID NIH HHS AI109891NIAID NIH HHS R43 AI109891NIAID NIH HHS R44 AI109891
6 · The paper itself

Abstract

Pertussis is a highly contagious disease for which prompt, point-of-care diagnosis remains an unmet clinical need. Results from conventional test modalities (nucleic acid detection, serology, and culture) take hours to days. To overcome this challenge, we identified a new biomarker (tracheal colonization factor A, TcfA) for detection of Bordetella pertussis infection by lateral flow immunoassay (LFIA). We developed a library of 28 epitope-mapped monoclonal antibodies against TcfA and incorporated three antibodies into a LFIA. The LFIA did not cross-react with common bacterial or fungal organisms, but did react with nine distinct B. pertussis strains. The minimal linear epitope sequences targeted by the LFIA were conserved in 98% of 954 B. pertussis isolates collected across 12 countries from 1949-2017. The LFIA's limit of detection was 3.0 × 10

Indexed as

Bordetella pertussisAnimalsAntibodies, MonoclonalBacterial ProteinsBiomarkersBuffersEpitope MappingHumansImmunoassayLimit of DetectionMiceNasopharynxPapioRabbitsSensitivity and SpecificityVirulence Factors, BordetellaAntibodies, MonoclonalBacterial ProteinsBiomarkersBufferstracheal colonization factor, Bordetella pertussisVirulence Factors, Bordetella

Identifiers

PMID32929160
PMCPMC7490692
OpenAlexW3084807890

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.