ArticleScientific reports2020
Evaluation of whole genome amplification and bioinformatic methods for the characterization of Leishmania genomes at a single cell level.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Unravelling drug resistance in leishmaniasis: genomic adaptations and emerging therapies.Frontiers in molecular biosciences · 2025Review
- A novel strain of Leishmania braziliensis harbors not a toti- but a bunyavirus.PLoS neglected tropical diseases · 2024Article
- Genomic Insight of Leishmania Parasite: In-Depth Review of Drug Resistance Mechanisms and Genetic Mutations.ACS omega · 2024Review
- Visceral Leishmaniasis Caused by Leishmania Tropica.Acta parasitologica · 2023Article
- Life in plastic, it's fantastic! HowFrontiers in cellular and infection microbiology · 2023Review
- Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods.Parasites & vectors · 2022Review
- Interspecies and Intrastrain Interplay amongMicroorganisms · 2022Review
- Dual-layered hydrogels allow complete genome recovery with nucleic acid cytometry.Biotechnology journal · 2022Article
- High throughput single-cell genome sequencing gives insights into the generation and evolution of mosaic aneuploidy in Leishmania donovani.Nucleic acids research · 2022Article
- Advances in application of single-cell RNA sequencing in cardiovascular research.Frontiers in cardiovascular medicine · 2022Review
- Impact of Genetic Diversity and Genome Plasticity ofFrontiers in cellular and infection microbiology · 2022Review
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?Pathogens (Basel, Switzerland) · 2021Review
- Single-cell genome sequencing of protozoan parasites.Trends in parasitology · 2021Review
- Downregulation of FeSOD-A expression in Leishmania infantum alters trivalent antimony and miltefosine susceptibility.Parasites & vectors · 2021Article
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Authors and funding
10 authors.
Funding
Abstract
Here, we report a pilot study paving the way for further single cell genomics studies in Leishmania. First, the performances of two commercially available kits for Whole Genome Amplification (WGA), PicoPLEX and RepliG were compared on small amounts of Leishmania donovani DNA, testing their ability to preserve specific genetic variations, including aneuploidy levels and SNPs. We show here that the choice of WGA method should be determined by the planned downstream genetic analysis, PicoPLEX and RepliG performing better for aneuploidy and SNP calling, respectively. This comparison allowed us to evaluate and optimize corresponding bio-informatic methods. As PicoPLEX was shown to be the preferred method for studying single cell aneuploidy, this method was applied in a second step, on single cells of L. braziliensis, which were sorted by fluorescence activated cell sorting (FACS). Even sequencing depth was achieved in 28 single cells, allowing accurate somy estimation. A dominant karyotype with three aneuploid chromosomes was observed in 25 cells, while two different minor karyotypes were observed in the other cells. Our method thus allowed the detection of aneuploidy mosaicism, and provides a solid basis which can be further refined to concur with higher-throughput single cell genomic methods.
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