ArticleScientific reports2020
Cultural isolation of spore-forming bacteria in human feces using bile acids.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Metabolic pathways associated with Firmicutes prevalence in the gut of multiple livestock animals and humans.Animal microbiome · 2025Article
- Metagenome-guided culturomics for the targeted enrichment of gut microbes.Nature communications · 2025Article
- Bile acids as germinants for Clostridioides difficile spores, evidence of adaptation to the gut?FEMS microbiology reviews · 2025Review
- Bile acid is a responsible host factor for high-fat diet-induced gut microbiota alterations in rats: proof of the "bile acid hypothesis".Bioscience of microbiota, food and health · 2025Article
- Rich microbial and depolymerising diversity in Antarctic krill gut.Microbiology spectrum · 2024Article
- Isolation of potentially novel species expands the genomic and functional diversity of Lachnospiraceae.iMeta · 2024Article
- Culture-dependent screening of endospore-forming clostridia in infant feces.BMC microbiology · 2023Article
- Isolation and Identification of Bacteria of GenusMicroorganisms · 2023Article
- CRISPR-Cas provides limited phage immunity to a prevalent gut bacterium in gnotobiotic mice.The ISME journal · 2023Article
- Mechanisms and Applications of Bacterial Sporulation and Germination in the Intestine.International journal of molecular sciences · 2022Review
- Host adaptation in gut Firmicutes is associated with sporulation loss and altered transmission cycle.Genome biology · 2021Article
- Cultivation of Spore-Forming Gut Microbes Using a Combination of Bile Acids and Amino Acids.Microorganisms · 2021Article
- Ecological Adaptations of Gut Microbiota Members and Their Consequences for Use as a New Generation of Probiotics.International journal of molecular sciences · 2021Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Structurally-diversified bile acids (BAs) are involved in shaping of intestinal microbiota as well as absorption of dietary lipids. Taurocholic acid, a conjugated form of BA, has been reported to be a factor triggering germination of a wide range of spore-forming bacteria in intestine. To test a hypothesis that other BAs also promote germination of intestinal bacteria, we attempted culture of bacteria from ethanol-treated feces by using a series of BAs. It was found that conjugated-BAs, notably three glycine-conjugated BAs, glycodeoxycholic acid and glycochenodeoxycholic acid, significantly increased the number and the species variety of colonies formed on the agar plate. These colonized bacteria mostly belonged to class Clostridia, mainly consisting of families Lachnospiraceae, Clostridiaceae, and Peptostreptococcaceae. There were several types of bacteria associated with different sensitivity to each BA. Eventually, we isolated 72 bacterial species of which 61 are known and 11 novel. These results demonstrate that the culturable range of bacteria in intestine can be widened using the germination-inducing activity of BAs. This approach would advance the research on spore-forming Clostridia that contains important but difficult-to-cultured bacteria associate with host health and diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.