ArticleVeterinary research2020
Acute and long-term effects of antibiotics commonly used in laboratory animal medicine on the fecal microbiota.
Article in Veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Efficacy and Impact of Prophylactic Compared with Symptomatic Amoxicillin, Azithromycin, or Trimethoprim/Sulfamethoxazole Administration on Corynebacterium bovis Infection in NSG Mice (Mus musculus).Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Isolation, Characterization, and Epizootiology of Clostridioides cuniculi from Immunodeficient Mice with Enteric Disease.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Assessment of Antimicrobial Therapy in Eradicating Chlamydia muridarum in Research Mice: Immune Status and Its Impact on Outcomes.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Effects of antibiotic cocktail on the fecal microbiota and their potential correlation of local immune response.BMC microbiology · 2024Article
- Exploring Gut Microbiota Alterations with Trimethoprim-Sulfamethoxazole and Dexamethasone in a Humanized Microbiome Mouse Model.Microorganisms · 2024Article
- Infection and antibiotic-associated changes in the fecal microbiota ofAntimicrobial agents and chemotherapy · 2024Article
- Temporal dynamics of the fecal microbiome in female pigs from early life through estrus, parturition, and weaning of the first litter of piglets.Animal microbiome · 2024Article
- Age influences the temporal dynamics of microbiome and antimicrobial resistance genes among fecal bacteria in a cohort of production pigs.Animal microbiome · 2023Article
- A longitudinal investigation of the effects of age, dietary fiber type and level, and injectable antimicrobials on the fecal microbiome and antimicrobial resistance of finisher pigs.Journal of animal science · 2022Article
- Reduced housing density improves statistical power of murine gut microbiota studies.Cell reports · 2022Article
- Evolution of the murine gut resistome following broad-spectrum antibiotic treatment.Nature communications · 2022Article
- The gut microbiome of laboratory mice: considerations and best practices for translational research.Mammalian genome : official journal of the International Mammalian Genome Society · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Biomedical research relies on the use of animal models, and the animals used in those models receive medical care, including antibiotics for brief periods of time to treat conditions such as dermatitis, fight wounds, and suspected bacterial pathogens of unknown etiology. As many mouse model phenotypes are sensitive to changes in the gut microbiota, our goal was to examine the effect of antibiotics commonly administered to mice. Therefore, four treatment groups (subcutaneous enrofloxacin for 7 days, oral enrofloxacin for 14 days, oral trimethoprim-sulfamethoxazole for 14 days, and topical triple antibiotic ointment for 14 days) alongside a fifth control group receiving no treatment (n = 12/group) were included in our study. Fecal samples were collected prior to treatment, immediately after two weeks of exposure, and four weeks after cessation of treatment, and subjected to 16S rRNA library sequencing. The entire experimental design was replicated in mice from two different suppliers. As expected, several treatments including enrofloxacin and triple antibiotic ointment substantially decreased the amount of DNA recovered from fecal material, as well as the microbial richness. Notably, many of these effects were long-lasting with diminished gut microbiota (GM) richness four weeks following exposure, in both substrains of mice. Trimethoprim-sulfamethoxazole induced minimal to no discernible changes in the taxonomic composition beyond that seen in control mice. Collectively, these data highlight the need to consider the impact on GM of brief and seemingly routine use of antibiotics in the clinical care of research animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.