Intracellular Ser/Thr/Tyr phosphoproteome of the oral commensal Streptococcus gordonii DL1.

Carolina Robertsson, Gunnel Svensäter, Zoltan Blum, Claes Wickström

Open access · goldAbstract read

In one paragraph

Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.3field-weighted citation impact, top 40% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

Proteomic response inJournal of oral microbiology · 2021
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Carolina RobertssonDepartment of Oral Biology and Pathology, Faculty of Odontology, Malmö University, 20506, Malmö, Sweden. carolina.robertsson@mau.se.ORCID 0000-0001-9545-9161

Gunnel SvensäterDepartment of Oral Biology and Pathology, Faculty of Odontology, Malmö University, 20506, Malmö, Sweden.

Zoltan BlumDepartment of Biomedical Science, Malmö University, 20506, Malmö, Sweden.

Claes WickströmDepartment of Oral Biology and Pathology, Faculty of Odontology, Malmö University, 20506, Malmö, Sweden.

Malmö University · SE

Funding

Malmö Högskola FORESIGHTVetenskapsrådet 201601994

6 · The paper itself

Abstract

backgroundTo respond and adapt to environmental challenges, prokaryotes regulate cellular processes rapidly and reversibly through protein phosphorylation and dephosphorylation. This study investigates the intracellular proteome and Ser/Thr/Tyr phosphoproteome of the oral commensal Streptococcus gordonii. Intracellular proteins from planktonic cells of S. gordonii DL1 were extracted and subjected to 2D-gel electrophoresis. Proteins in general were visualized using Coomassie Brilliant Blue and T-Rex staining. Phosphorylated proteins were visualized with Pro-Q Diamond Phosphoprotein Gel Stain. Proteins were identified by LC-MS/MS and sequence analysis.

resultsIn total, sixty-one intracellular proteins were identified in S. gordonii DL1, many of which occurred at multiple isoelectric points. Nineteen of these proteins were present as one or more Ser/Thr/Tyr phosphorylated form. The identified phosphoproteins turned out to be involved in a variety of cellular processes.

conclusionNineteen phosphoproteins involved in various cellular functions were identified in S. gordonii. This is the first time the global intracellular Ser/Thr/Tyr phosphorylation profile has been analysed in an oral streptococcus. Comparison with phosphoproteomes of other species from previous studies showed many similarities. Proteins that are consistently found in a phosphorylated state across several species and growth conditions may represent a core phosphoproteome profile shared by many bacteria.

Indexed as

Bacterial ProteinsChromatography, LiquidElectrophoresis, Gel, Two-DimensionalHumansMouthPhosphoproteinsPhosphorylationSerineStreptococcus gordoniiTandem Mass SpectrometryThreonineTyrosineBacterial ProteinsPhosphoproteinsSerineThreonineTyrosine2DEOral bacteriaPhosphoproteomePro-Q diamondStreptococciStreptococcus gordonii

Identifiers

PMID32928109

DOI10.1186/s12866-020-01944-y

PMCPMC7488673

OpenAlexW3086830258

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.