ArticleNanomaterials (Basel, Switzerland)2020
Adsorption of Cellulase on Wrinkled Silica Nanoparticles with Enhanced Inter-Wrinkle Distance.
Article in Nanomaterials (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Enzymes for Biomass Pretreatment: A Comprehensive Review.Advances in biochemical engineering/biotechnology · 2025Review
- Nanomaterial scaffolds for enzymatic polymer degradation: a tool to advance current biodegradation assessments of polymers in liquid formulation.Bioscience nanotechnology · 2025Review
- Controlling the Adsorption of β-Glucosidase onto Wrinkled SiOLangmuir : the ACS journal of surfaces and colloids · 2023Article
- Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing.Nanomaterials (Basel, Switzerland) · 2022Review
- Enhancement of the Catalytic Performance and Operational Stability of Sol-Gel-Entrapped Cellulase by Tailoring the Matrix Structure and Properties.Gels (Basel, Switzerland) · 2022Article
- Improvement in the Thermostability of a Recombinant β-Glucosidase Immobilized in Zeolite under Different Conditions.Molecules (Basel, Switzerland) · 2022Article
- Solvent-Free One-Pot Synthesis of Epoxy Nanocomposites Containing Mg(OH)Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Co-immobilization of Cellulase and β-Glucosidase into Mesoporous Silica Nanoparticles for the Hydrolysis of Cellulose Extracted fromLangmuir : the ACS journal of surfaces and colloids · 2022Article
- Silica Meets Tannic Acid: Designing Green Nanoplatforms for Environment Preservation.Molecules (Basel, Switzerland) · 2022Article
- Biocatalytic System Made of 3D Chitin, Silica Nanopowder and Horseradish Peroxidase for the Removal of 17α-Ethinylestradiol: Determination of Process Efficiency and Degradation Mechanism.Molecules (Basel, Switzerland) · 2022Article
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6 authors.
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Abstract
Mesoporous silica materials offer a unique opportunity for enzyme immobilization thanks to their properties, such as tuneable pore size, large surface area and easy functionalization. However, a significant enhancement of cellulase enzyme activity entrapped inside the silica pores still represents a challenge. In this work, we immobilized cellulase by adsorption on wrinkled silica nanoparticles (WSNs), obtaining an active and stable biocatalyst. We used pentanol as co-solvent to synthesize WSNs with enhanced inter-wrinkle distance in order to improve cellulase hosting. The physical-chemical and morphological characterization of WSNs and cellulase/WSNs was performed by thermogravimetric (TG), Fourier transform infrared (FT-IR), and transmission electron microscopy (TEM) analyses. The obtained results showed that this matrix generates a favourable microenvironment for hosting cellulase. The results of the catalytic assays and operational stability confirmed the key role of size, morphology and distribution of the pores in the successful outcome of the cellulase immobilization process. The immobilization procedure used allowed preserving most of the secondary structure of the enzyme and, consequently, its catalytic activity. Moreover, the same value of glucose yield was observed for five consecutive runs, showing a high operational stability of the biocatalyst.
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