Evidence map›Paper›PMID 32926371›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

High-Dimensional Flow Cytometry Analysis of Regulatory Receptors on Human T Cells, NK Cells, and NKT Cells.

Ryosuke Nakagawa, Jason Brayer, Nicole Restrepo, James J Mulé, Adam W Mailloux

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ryosuke NakagawaDepartment of Immunology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
Jason BrayerDepartment of Malignant Hematology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
Nicole RestrepoDepartment of Immunology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
James J MuléDepartment of Immunology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
Adam W MaillouxDepartment of Immunology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA. adam.mailloux@moffitt.org.
Moffitt Cancer Center · US

Funding

TRANSLATIONAL RESEARCHP30CA076292 · NCI · UNIVERSITY OF SOUTH FLORIDA · PI KENNETH Lynn WRIGHT · 1998 to 2026
$93.5M
Tissue, Pathology and BioinformaticsP50CA168536 · NCI · H. LEE MOFFITT CANCER CTR & RES INST · PI MULE, JAMES J. · 2013 to 2017
$8.5M
Designing Lymph Nodes for Cancer ImmunotherapyR01CA148995 · NCI · H. LEE MOFFITT CANCER CTR & RES INST · PI MULE, JAMES J. · 2010 to 2014
$1.7M
NCI NIH HHS P30 CA076292NCI NIH HHS P50 CA168536NCI NIH HHS R01 CA148995
6 · The paper itself

Abstract

The field of flow cytometry has witnessed rapid technological advancements in the last few decades. While the founding principles of fluorescent detection on cells (or particles) within a uniform fluid stream remains largely unchanged, the availability more sensitive cytometers with the ability to multiplex more and more florescent signals has resulted in very complex high-order assays. This results in the co-use of fluorophores with increased levels of emission overlap and/or spillover spreading than in years past and thus requires careful and well thought out planning for flow cytometry assay development. As an example, we present the development of a large 18-color (20 parameter) flow cytometry assay designed to take an in depth analysis of effector lymphocyte phenotypes, with careful attention to assay controls and panel design.

Indexed as

AntibodiesFlow CytometryHumansImmune Checkpoint ProteinsImmunophenotypingKiller Cells, NaturalNatural Killer T-CellsStaining and LabelingAntibodiesImmune Checkpoint ProteinsAdaptive immune cellsImmune checkpointInnate immune cellsMemory markersNK cellNK receptorNKT cellT cell

Identifiers

PMID32926371
PMCPMC7744271
OpenAlexW3086059028

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.