ArticleJournal of visualized experiments : JoVE2020
Histological-Based Stainings using Free-Floating Tissue Sections.
Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
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- A Novel Mouse Model of Mixed Dementia Using Chronic Cerebral Hypoperfusion Induced by Bilateral Carotid Artery Stenosis.Journal of Korean medical science · 2025Article
- Short-term exposure to polystyrene microplastics alters cognition, immune, and metabolic markers in an apolipoprotein E (APOE) genotype and sex-dependent manner.Environmental research communications · 2025Article
- AI-enabled drug prediction and gene network analysis reveal therapeutic use of vorinostat for Rett Syndrome in preclinical models.Communications medicine · 2025Article
- Extracellular Matrix Alterations Due to Early-Life Adversity: Implications for Auditory Learning in Male Sprague-Dawley Rats.Molecular neurobiology · 2025Article
- Multiplex Immunofluorescent Batch Labeling of Marmoset Brain Sections.Brain and behavior · 2025Article
- Visualizing the Cellular and Subcellular Distribution of Fms-like Tyrosine Kinase 3 (Flt3) and Other Neuronal Proteins Using Alkaline Phosphatase (AP) Immunolabeling.International journal of molecular sciences · 2025Article
- Assessment and Quantification of Foam Cells and Lipid Droplet-Accumulating Microglia in Mouse Brain Tissue Using BODIPY Staining.Bio-protocol · 2024Article
- Assessing Different Histological Preparations for Reconstruction of Astrocyte Tridimensional Structure.Cells · 2024Article
- Fluorescent liposomal nanocarriers for targeted drug delivery in ischemic stroke therapy.Biomaterials science · 2023Article
- Acute Exposure to Microplastics Induced Changes in Behavior and Inflammation in Young and Old Mice.International journal of molecular sciences · 2023Article
- A novel immunohistochemical protocol for paraffin embedded tissue sections using free-floating techniques.Frontiers in neuroanatomy · 2023Article
- Colocalization of senescent biomarkers in deep, superficial, and ovarian endometriotic lesions: a pilot study.Scientific reports · 2022Article
- Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections.Journal of visualized experiments : JoVE · 2022Article
- Introducing immunohistochemistry to the molecular biology laboratory.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology · 2022Article
- Periodontal Infection Aggravates C1q-Mediated Microglial Activation and Synapse Pruning in Alzheimer's Mice.Frontiers in immunology · 2022Article
- Palmitoylethanolamide attenuates neurodevelopmental delay and early hippocampal damage following perinatal asphyxia in rats.Frontiers in behavioral neuroscience · 2022Article
- Free-floating Immunostaining of Mouse Brains.Journal of visualized experiments : JoVE · 2021Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Immunohistochemistry is a widely used technique to visualize specific tissue structures as well as protein expression and localization. Two alternative approaches are widely used to handle the tissue sections during the staining procedure, one approach consists of mounting the sections directly on glass slides, while a second approach, the free-floating, allows for fixed sections to be maintained and stained while suspended in solution. Although slide-mounted and free-floating approaches may yield similar results, the free-floating technique allows for better antibody penetration and thus should be the method of choice when thicker sections are to be used for 3D reconstruction of the tissues, for example when the focus of the experiment is to gain information on dendritic and axonal projections in brain regions. In addition, since the sections are kept in solution, a single aliquot can easily accommodate 30 to 40 sections, handling of which is less laborious, particularly in large-scale biomedical studies. Here, we illustrate how to apply the free-floating method to fluorescent immunohistochemistry staining, with a major focus on brain sections. We will also discuss how the free-floating technique can easily be modified to fit the individual needs of researchers and adapted to other tissues as well as other histochemical-based stainings, such as hematoxylin and eosin and cresyl violet, as long as tissue samples are properly fixed, typically with paraformaldehyde or formalin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.