Evidence map›Paper›PMID 32924474›Full record

ArticleJournal of the American Society for Mass Spectrometry2020

Streamlined Analysis of Cardiolipins in Prokaryotic and Eukaryotic Samples Using a Norharmane Matrix by MALDI-MSI.

Hyojik Yang, Shelley N Jackson, Amina S Woods, David R Goodlett, Robert K Ernst, Alison J Scott

Open access · greenAbstract read
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 22 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Quantitative mass spectrometry imaging: therapeutics & biomolecules.Chemical communications (Cambridge, England) · 2024
    Review
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 3 countries.

Hyojik YangDepartment of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore 21201, Maryland, United States.
Shelley N JacksonStructural Biology Core, NIDA IRP, NIH, Baltimore 21224, Maryland, United States.
Amina S WoodsStructural Biology Core, NIDA IRP, NIH, Baltimore 21224, Maryland, United States.ORCID http://orcid.org/0000-0002-9716-345X
David R GoodlettDepartment of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore 21201, Maryland, United States.
Robert K ErnstDepartment of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore 21201, Maryland, United States.
Alison J ScottDepartment of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore 21201, Maryland, United States.ORCID http://orcid.org/0000-0001-6969-4707
University of Maryland, Baltimore · USNational Institute on Drug Abuse · US

Funding

MS Diagnostic Bacterial Identification LibraryR01AI147314 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI ERNST, ROBERT K, GOODLETT, DAVID ROBINSON · 2020 to 2024
$2.3M
MS diagnostic bacterial identification libraryR01GM111066 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI ERNST, ROBERT K, GOODLETT, DAVID ROBINSON · 2014 to 2017
$1.2M
Intramural NIH HHS Z99 DA999999NIAID NIH HHS R01 AI147314NIGMS NIH HHS R01 GM111066
6 · The paper itself

Abstract

Cardiolipins (CLs) are an important, regulated lipid class both in prokaryotic and eukaryotic cells, yet they remain largely unexplored by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) in tissues. To date, no in-depth optimization studies of label-free visualization of CLs in complex biological samples have been reported. Here we report a streamlined modification to our previously reported MALDI-MSI method for detection of endogenous CLs in prokaryotic and eukaryotic cells based on preparation with norharmane (NRM) matrix. Notably, the use of NRM matrix permitted sensitive detection (4.7 pg/mm

Indexed as

AnimalsBacteriaCarbolinesCardiolipinsMiceSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationCarbolinesCardiolipinsnorharmancardiolipineukaryotic lipidsMALDImass spectrometry imagingnorharmane (NRM)prokaryotic lipids

Identifiers

PMID32924474
PMCPMC8681877
OpenAlexW3084766125

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.